Methods and Protocols

  • Qunwei Zhang

Part of the Methods in Molecular Biology book series (MIMB, volume 1894)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Dustin T. Savage, J. Zach Hilt, Thomas D. Dziubla
    Pages 1-29
  3. Yiling Hong, Nymph Chan, Aynun N. Begum
    Pages 57-72
  4. Maria Dusinska, Espen Mariussen, Elise Rundén-Pran, Alexandra Misci Hudecova, Elisabeth Elje, Alena Kazimirova et al.
    Pages 83-122
  5. Yue Zhang, Rong Wan, Qunwei Zhang, Yiqun Mo
    Pages 133-143
  6. Rong Wan, Yiqun Mo, Ruirui Tong, Meiqin Gao, Qunwei Zhang
    Pages 145-159
  7. Gao Long, Yiqun Mo, Qunwei Zhang, Mizu Jiang
    Pages 161-169
  8. Lingfang Feng, Jianlin Lou
    Pages 181-227
  9. Fuli Zheng, Huangyuan Li
    Pages 229-246
  10. Junzhe Zhang, Xiao He, Michael T. Tseng
    Pages 247-269
  11. Sameera Nallanthighal, Ramune Reliene
    Pages 301-312
  12. Yiqun Mo, Yue Zhang, Qunwei Zhang
    Pages 313-322
  13. Gary W. Hoyle, Connie F. Schlueter, Sadiatu Musah
    Pages 323-330
  14. Eduard Dumitrescu, Kenneth Wallace, Silvana Andreescu
    Pages 331-343
  15. Zhizhen Xu, Yingying Liu, Yuqian Wang
    Pages 345-352
  16. Peng Zhao, Yuanbao Zhang
    Pages 353-367
  17. Back Matter
    Pages 369-371

About this book


This volume explores the latest techniques used to investigate nanotoxicity. The protocols covered in this book range from routine nanotoxicity assays to genomic and proteomic approaches in vivo and in vitro. The chapters discuss specific topics such as preparation and utilization of 3D human liver microtissue models; assessment of ovarian granulosa cells with exposure to nanoparticles; DNA methylation analysis; evaluation of genotoxicity of nanoparticles in mouse models; and studying nanotoxicity using embryonic zebrafish. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Nanotoxicity: Methods and Protocols is a valuable resource for researchers and postgraduate students in the fields of toxicology, environmental science, pharmacological sciences, and clinical medicine. This volume also serves as a starting point for researchers interested in learning more about this developing field.


Nanotoxicology Nanotechnology Nanoparticles Pharmacological science Metabolomics biotechnology

Editors and affiliations

  • Qunwei Zhang
    • 1
  1. 1.Department of Environmental and Occupational Health Sciences, School of Public Health and Information SciencesUniversity of LouisvilleLouisvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8915-7
  • Online ISBN 978-1-4939-8916-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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