Cancer Immunosurveillance

Methods and Protocols

  • Alejandro López-Soto
  • Alicia R. Folgueras

Part of the Methods in Molecular Biology book series (MIMB, volume 1884)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Willem H. Zoutman, Rogier J. Nell, Pieter A. van der Velden
    Pages 1-14
  3. Imran Uddin, Kroopa Joshi, Theres Oakes, James M. Heather, Charles Swanton, on behalf of the TRACERx consortium et al.
    Pages 15-42
  4. Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, Jianxun Song
    Pages 43-55
  5. Akshat Sharma, Nicholas A. Zumwalde, Jenny E. Gumperz
    Pages 57-72
  6. Monica Parodi, Raffaella Meazza, Chiara Vitale, Gabriella Pietra, Paolo Carrega, Massimo Vitale
    Pages 87-117
  7. Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez, Alejandro López-Soto
    Pages 119-130
  8. Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo González, Alejandro López-Soto
    Pages 131-139
  9. Marina Wierz, Sandrine Pierson, Ernesto Gargiulo, Coralie Guerin, Etienne Moussay, Jerome Paggetti
    Pages 231-245
  10. Chiara Nicolazzo, Angela Gradilone, Guido Carpino, Paola Gazzaniga, Cristina Raimondi
    Pages 247-258
  11. Anat Biran, Ziv Porat, Valery Krizhanovsky
    Pages 259-267
  12. Xiuyun Jiang, Y. David Seo, Kevin M. Sullivan, Venu G. Pillarisetty
    Pages 283-295
  13. Juliette Humeau, Sarah Lévesque, Guido Kroemer, Jonathan G. Pol
    Pages 297-315
  14. Annunziata Nigro, Barbara Montico, Vincenzo Casolaro, Jessica Dal Col
    Pages 317-333
  15. Yuan He, Wijnand Helfrich, Edwin Bremer
    Pages 335-347
  16. Qumiao Xu, Hizkia Harto, Robert Berahovich, Shirley Xu, Hua Zhou, Vita Golubovskaya et al.
    Pages 349-360
  17. Back Matter
    Pages 361-363

About this book


This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field.


Oncoimmunology immune receptors NK cell-mediated cytotoxicity tumor T cell exhaustion

Editors and affiliations

  • Alejandro López-Soto
    • 1
  • Alicia R. Folgueras
    • 2
  1. 1.Departamento de Biología Funcional, InmunologíaUniversidad de Oviedo, Instituto Universitario de Oncología del Principado de Asturias (IUOPA)OviedoSpain
  2. 2.Departamento de Bioquímica y Biología MolecularUniversidad de Oviedo, Instituto Universitario de Oncología del Principado de Asturias (IUOPA)OviedoSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8884-6
  • Online ISBN 978-1-4939-8885-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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