Methods and Protocols

  • Yilin Hu

Part of the Methods in Molecular Biology book series (MIMB, volume 1876)

Table of contents

  1. Front Matter
    Pages i-x
  2. Metalloproteins

    1. Front Matter
      Pages 1-1
    2. Nathaniel S. Sickerman, Yilin Hu, Markus W. Ribbe
      Pages 3-24
    3. Jae-Hun Jeoung, Berta M. Martins, Holger Dobbek
      Pages 37-54
    4. Dimitri Niks, Russ Hille
      Pages 55-63
    5. Nathaniel S. Sickerman, Yilin Hu
      Pages 65-88
  3. Genetic and Biochemical Methods

    1. Front Matter
      Pages 89-89
    2. Chi-Chung Lee, Markus W. Ribbe, Yilin Hu
      Pages 111-124
    3. Jürgen Moser, Jan Jasper, José Vazquez Ramos, Sven T. Sowa, Gunhild Layer
      Pages 125-140
    4. Paul Kaufmann, Chantal Iobbi-Nivol, Silke Leimkühler
      Pages 141-152
  4. Structural and Spectroscopic Methods

    1. Front Matter
      Pages 153-153
    2. Belinda B. Wenke, Renee J. Arias, Thomas Spatzal
      Pages 155-165
    3. Jae-Hun Jeoung, Berta M. Martins, Holger Dobbek
      Pages 167-178
    4. Andrew Jasniewski, Yilin Hu, Markus W. Ribbe
      Pages 197-211
  5. Chemical Methods and Theoretical Calculations

    1. Front Matter
      Pages 227-227
    2. Martin T. Stiebritz, Yilin Hu
      Pages 245-266
  6. Back Matter
    Pages 267-275

About this book


This volume provides an up-to-date, in-depth overview of the methods that have been applied to studying the complex metalloproteins at a molecular level. Chapters cover a wide range of approaches focusing on genetic, biochemical, spectroscopic, chemical methods, and theoretical calculations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Metalloproteins: Methods and Protocols aims to be useful for anyone who is interested in metalloprotein research and wants to address the unanswered mechanistic and biosynthetic questions of these fascinating enzyme systems.


Nitrogenase Nitrogen Fixation Carbon Monoxide Dehydrogenase ENDOR Spectroscopy Spectroscopy

Editors and affiliations

  • Yilin Hu
    • 1
  1. 1.Department of Molecular Biology and BiochemistryUniversity of California-IrvineIrvineUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8863-1
  • Online ISBN 978-1-4939-8864-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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