© 2019

BCL-2 Family Proteins

Methods and Protocols

  • Evripidis Gavathiotis

Part of the Methods in Molecular Biology book series (MIMB, volume 1877)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Jason D. Huska, Heather M. Lamb, J. Marie Hardwick
    Pages 1-21
  3. Abdel Aouacheria, Vincent Navratil, Christophe Combet
    Pages 23-43
  4. Jonathan Lopez, Stephen W. G. Tait
    Pages 45-60
  5. Cameron Fraser, Jeremy Ryan, Kristopher Sarosiek
    Pages 61-76
  6. Mark P. A. Luna-Vargas, Jarvier N. Mohammed, Jesse D. Gelles, Jerry Edward Chipuk
    Pages 121-130
  7. Jialing Lin, Arthur E. Johnson, Zhi Zhang
    Pages 131-149
  8. Joachim Lauterwasser, Franziska Fimm-Todt, Frank Edlich
    Pages 151-161
  9. Morey L. Smith, Stephen K. Tahir
    Pages 163-172
  10. Marcos A. Carpio, Samuel G. Katz
    Pages 173-183
  11. Thomas P. Garner, Evripidis Gavathiotis
    Pages 217-231
  12. Elizabeth J. Osterlund, Nehad Hirmiz, Christian Tardif, David W. Andrews
    Pages 305-335
  13. Back Matter
    Pages 359-360

About this book


This volume explores numerous techniques for the genetic, molecular, biochemical, and structural examination of BCL-2 family proteins and their interactions. The chapters in this book cover topics such as the relevance of BCL-2 proteins in health and disease; evaluating cellular dependencies to specific BCL-2 family proteins; flow-cytometry-based methods for measuring BCL-2 proteins and mitochondrial-based cell death; measuring activity and  interactions of BCL-2 family proteins in the presence of mitochondria, artificial membranes or yeast; conformational activation and oligomerization of pro-apoptotic proteins BAX and BAK leading to cytochrome c release and apoptosis; structural and biophysical studies in solution and lipid vesicles using nuclear magnetic resonance, cryo-electron microscopy, fluorescence microscopy and electron paramagnetic resonance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, BCL-2 Family Proteins: Methods and Protocols is a valuable resource to inspire and encourage novice and established scientists to further their research and make new discoveries in this exciting field.


homology motif apoptosis C. elegans Drosophila autophagy flow-cytometry BAX pores BAK pores

Editors and affiliations

  • Evripidis Gavathiotis
    • 1
  1. 1.Department of BiochemistryAlbert Einstein College of MedicineBronxUSA

Bibliographic information

  • Book Title BCL-2 Family Proteins
  • Book Subtitle Methods and Protocols
  • Editors Evripidis Gavathiotis
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-8860-0
  • eBook ISBN 978-1-4939-8861-7
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XI, 360
  • Number of Illustrations 30 b/w illustrations, 66 illustrations in colour
  • Topics Protein Science
  • Buy this book on publisher's site
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