Brain Tumor Stem Cells

Methods and Protocols

  • Sheila K. Singh
  • Chitra Venugopal

Part of the Methods in Molecular Biology book series (MIMB, volume 1869)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Nicolas Yelle, David Bakhshinyan, Chitra Venugopal, Sheila K. Singh
    Pages 1-9
  3. Charles Chesnelong, Ian Restall, Samuel Weiss
    Pages 11-21
  4. Sara Badodi, Silvia Marino, Loredana Guglielmi
    Pages 23-36
  5. Scott Ryall, Anthony Arnoldo, Javal Sheth, Sheila K. Singh, Cynthia Hawkins
    Pages 57-67
  6. Minomi K. Subapanditha, Ashley A. Adile, Chitra Venugopal, Sheila K. Singh
    Pages 69-77
  7. Mathieu Seyfrid, David Bobrowski, David Bakhshinyan, Nazanin Tatari, Chitra Venugopal, Sheila K. Singh
    Pages 79-84
  8. Michelle M. Kameda-Smith, Minomi K. Subapanditha, Sabra K. Salim, Chitra Venugopal, Sheila K. Singh
    Pages 85-91
  9. Montserrat Lara-Velazquez, Rawan Al-kharboosh, Luis Prieto, Paula Schiapparelli, Alfredo Quiñones-Hinojosa
    Pages 93-104
  10. Rawan Al-kharboosh, Montserrat Lara-Velazquez, Luis Prieto, Rachel Sarabia-Estrada, Alfredo Quiñones-Hinojosa
    Pages 105-116
  11. Ingrid Qemo, Lisa A. Porter
    Pages 117-126
  12. Ludivine Coudière Morrison, Nazanin Tatari, Tamra E. Werbowetski-Ogilvie
    Pages 127-142
  13. Hunter McColl, Jamie L. Zagozewski, David D. Eisenstat
    Pages 143-153
  14. Amy X. Hu, Jarrett J. Adams, Parvez Vora, Maleeha Qazi, Sheila K. Singh, Jason Moffat et al.
    Pages 155-168
  15. Michael Aregger, Megha Chandrashekhar, Amy Hin Yan Tong, Katherine Chan, Jason Moffat
    Pages 169-188
  16. Ashley A. Adile, David Bakhshinyan, Chitra Venugopal, Sheila K. Singh
    Pages 189-196
  17. Elena Binda, Alberto Visioli, Nadia Trivieri, Angelo Luigi Vescovi
    Pages 197-205
  18. Mohini Singh, Neil Savage, Sheila K. Singh
    Pages 231-238
  19. Katie M. Parkins, Ashley V. Makela, Amanda M. Hamilton, Paula J. Foster
    Pages 239-251
  20. Back Matter
    Pages 253-254

About this book


This detailed volume compiles the best methodologies and experimental techniques to profile and extract maximal data from brain tumor stem cells (BTSCs), the experimental paradigm for brain cancer research that offers insights into cancer stem cell populations that may drive not only tumor initiation but tumor recurrence and patient relapse. The BTSC model recapitulates scientific observations made in brain cancer patients, and these chapters provide the reader with a comprehensive understanding of the skills and techniques that will unlock data from this most informative subset of cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Brain Tumor Stem Cells: Methods and Protocols serves as an ideal guide for researchers seeking to better understand the complexities of brain cancer.


Brain cancer BTSC research Human cancer stem cell (CSC) populations Therapeutic options Glioblastoma

Editors and affiliations

  • Sheila K. Singh
    • 1
  • Chitra Venugopal
    • 2
  1. 1.Stem Cell and Cancer Research InstituteMcMaster UniversityHamiltonCanada
  2. 2.Stem Cell and Cancer Research InstituteMcMaster UniversityHamiltonCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8804-4
  • Online ISBN 978-1-4939-8805-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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