Zinc Finger Proteins

Methods and Protocols

  • Jia Liu

Part of the Methods in Molecular Biology book series (MIMB, volume 1867)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Basic Biology and Engineering of ZFPs

    1. Front Matter
      Pages 1-1
    2. Hiroaki Taniguchi, Kohzoh Imai
      Pages 3-13
    3. Simona De Franco, Mitchell R. O’Connell, Marylène Vandevenne
      Pages 57-74
    4. Jia Liu, Jiangmei Li, Jie Li, Lianhui Zhu, Shaojie Wang, Xuan Wei et al.
      Pages 113-123
    5. Krissanapong Manotham, Supreecha Chattong
      Pages 125-138
  3. Methods for the Evaluation and Prevention of ZFN Cytotoxicity

    1. Front Matter
      Pages 139-139
    2. Jaichandran Sivalingam, Dimitar Kenanov, Wai Har Ng, Sze Sing Lee, Toan Thang Phan, Sebastian Maurer-Stroh et al.
      Pages 141-164
    3. Maui Nishio, Ayana Yamagishi, Kaori Tsukakoshi, Yoshio Kato, Chikashi Nakamura, Kazunori Ikebukuro
      Pages 165-174
    4. Jiani Xing, Cunfang Zhang, Kun Xu, Linyong Hu, Ling Wang, Tingting Zhang et al.
      Pages 175-183
  4. Delivery Methods of ZFPs

    1. Front Matter
      Pages 185-185
    2. Rex A. Dunham, Ahmed Elaswad, Zhenkui Qin
      Pages 201-214
    3. Beverly J. McCann, Andy Cox, Payam A. Gammage, James B. Stewart, Magdalena Zernicka-Goetz, Michal Minczuk
      Pages 215-228
    4. Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, Elizabeth A. Heller
      Pages 229-238
    5. Chonghua Ren, Alexa N. Adams, Benjamin Pyles, Barbara J. Bailus, Henriette O’Geen, David J. Segal
      Pages 239-251
    6. Jia Liu, Ya-jun Liang, Pei-ling Ren, Thomas Gaj
      Pages 253-273
  5. Back Matter
    Pages 275-276

About this book


This detailed volume explores the notable progress in the field of zinc finger proteins (ZFP) study through widely used methods and protocols involving their biological functions and applications. Beginning with a section on the basic biology of ZFPs and design and applications of custom ZFPs, the book continues by covering methods for the evaluation and prevention of ZFN-mediated cytotoxicity as well as a collection of the representative methods of ZFN delivery. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Zinc Finger Proteins: Methods and Protocols aims to prompt both the understanding of ZFP biology and the development of next-generation ZFP therapeutics.


ZFP domains Genome sequence targeting Gene expression Genomic engineering Delivery methods ZFN-induced genotoxicity Next-generation ZFP therapeutics

Editors and affiliations

  • Jia Liu
    • 1
  1. 1.Shanghai Institute for Advanced Immunochemical StudiesShanghaiTech UniversityShanghaiChina

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8798-6
  • Online ISBN 978-1-4939-8799-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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