Electrophoretic Separation of Proteins

Methods and Protocols

  • Biji T. Kurien
  • R. Hal Scofield

Part of the Methods in Molecular Biology book series (MIMB, volume 1855)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Pothur R. Srinivas
    Pages 23-29
  3. Ramesh Kumar, Wilbert A. Derbigny
    Pages 81-85
  4. Claudia Arndt, Stefanie Koristka, Anja Feldmann, Michael Bachmann
    Pages 87-91
  5. Biji T. Kurien, R. Hal Scofield
    Pages 93-100
  6. Hiroyuki Matsumoto, Hisao Haniu, Naoka Komori
    Pages 101-105
  7. Hiroyuki Matsumoto, Hisao Haniu, Biji T. Kurien, Naoka Komori
    Pages 107-113
  8. Engelbert Buxbaum
    Pages 115-124
  9. Christian Reichel, Günter Gmeiner, Philipp Reihlen, Mario Thevis, Wilhelm Schänzer
    Pages 131-149
  10. Syed R. Haider, Helen J. Reid, Barry L. Sharp
    Pages 151-160
  11. Marta P. Pereira Morais, Omar Kassaar, Stephen E. Flower, Robert J. Williams, Tony D. James, Jean M. H. van den Elsen
    Pages 161-175
  12. Henghang Tsai, Hon-Chiu Eastwood Leung
    Pages 211-227
  13. Malachi Blundon, Vinitha Ganesan, Brendan Redler, Phu T. Van, Jonathan S. Minden
    Pages 229-247
  14. Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, Jose Luis Rosa
    Pages 269-277
  15. Hiroyuki Matsumoto, Nobuaki Takemori, Naoka Komori
    Pages 287-289
  16. Mustafa Çelebier, Clara Ibáñez, Carolina Simó, Alejandro Cifuentes
    Pages 303-313
  17. Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, Jean-Louis Viovy
    Pages 327-340
  18. Karen A. Lewis, Sarah E. Altschuler, Deborah S. Wuttke
    Pages 341-354
  19. Jennifer A. Ream, L. Kevin Lewis, Karen A. Lewis
    Pages 363-370
  20. Bao-Shiang Lee, Lasanthi P. Jayathilaka, Jin-Sheng Huang, Shalini Gupta
    Pages 371-385
  21. Kim Y. C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, Peter Hoffmann
    Pages 403-415
  22. Stefanie Koristka, Claudia Arndt, Ralf Bergmann, Michael Bachmann
    Pages 461-465
  23. Biji T. Kurien, Rachna Aggarwal, R. Hal Scofield
    Pages 479-482
  24. Biji T. Kurien, R. Hal Scofield
    Pages 511-518
  25. Back Matter
    Pages 519-523

About this book


This volume expands upon Protein Electrophoresis (2012) and provides readers with easy-to-follow and reproducible methods to study electrophoresis. The chapters in this book cover topics such as the Cydex Blue assay; cellulose-acetate electrophoresis of hemoglobin; cationic electrophoresis; tricine-SDS-Page; identification of proteins on archived 2-D gels; cell surface protein biotinylation of SDS-PAGE analysis; and artifacts and common errors in protein gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and thorough, Electrophoretic Separation of Proteins: Methods and Protocols is a valuable resource for researchers who are interested in learning and experimenting with this field.


gel-electrophoresis homogenization Lysis Buffer PAGE PEGylated Proteins

Editors and affiliations

  • Biji T. Kurien
    • 1
  • R. Hal Scofield
    • 2
  1. 1.Department of MedicineUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  2. 2.Department of MedicineUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8792-4
  • Online ISBN 978-1-4939-8793-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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