Methods and Protocols

  • Kris Vleminckx

Part of the Methods in Molecular Biology book series (MIMB, volume 1865)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sean McNamara, Marcin Wlizla, Marko E. Horb
    Pages 1-17
  3. Marcin Wlizla, Sean McNamara, Marko E. Horb
    Pages 19-32
  4. Thomas Naert, Kris Vleminckx
    Pages 67-82
  5. Wouter Steyaert, Annekatrien Boel, Paul Coucke, Andy Willaert
    Pages 83-90
  6. Ken-ich T. Suzuki, Yuto Sakane, Miyuki Suzuki, Takashi Yamamoto
    Pages 91-103
  7. Hovy Ho-Wai Wong, Christine E. Holt
    Pages 119-131
  8. Albert Chesneau, Odile Bronchain, Muriel Perron
    Pages 133-146
  9. Engin Deniz, Emily K. Mis, Maura Lane, Mustafa K. Khokha
    Pages 163-174
  10. Meera Gupta, Matthew Sonnett, Lillia Ryazanova, Marc Presler, Martin Wühr
    Pages 175-194
  11. Matthew Sonnett, Meera Gupta, Thao Nguyen, Martin Wühr
    Pages 195-215
  12. Lukas Weiss, Thomas Offner, Thomas Hassenklöver, Ivan Manzini
    Pages 217-231
  13. Pierre Affaticati, Sébastien Le Mével, Arnim Jenett, Laurie Rivière, Elodie Machado, Bilal B. Mughal et al.
    Pages 233-241
  14. Jacques Robert, Eva-Stina Edholm, Francisco De Jesus Andino
    Pages 265-273
  15. Back Matter
    Pages 301-302

About this book


This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Xenopus: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping Xenopus at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model.


CRISPR Cas9 X. tropicalis TALEN Frog models

Editors and affiliations

  • Kris Vleminckx
    • 1
  1. 1.Department of Biomedical Molecular BiologyGhent UniversityGhentBelgium

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8783-2
  • Online ISBN 978-1-4939-8784-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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