Insect Genomics

Methods and Protocols

  • Susan J. Brown
  • Michael E. Pfrender

Part of the Methods in Molecular Biology book series (MIMB, volume 1858)

Table of contents

  1. Front Matter
    Pages i-xi
  2. J. Spencer Johnston, Angelina Bernardini, Carl E. Hjelmen
    Pages 15-26
  3. Susan J. Brown, Michelle Coleman
    Pages 27-32
  4. Xiaolong Cao, Haobo Jiang
    Pages 45-57
  5. Robert M. Waterhouse, Mathieu Seppey, Felipe A. Simão, Evgeny M. Zdobnov
    Pages 59-74
  6. Mei-Ju May Chen, Han Lin, Li-Mei Chiang, Christopher P. Childers, Monica F. Poelchau
    Pages 75-87
  7. R. Taylor Raborn, Volker P. Brendel
    Pages 99-116
  8. Majid Kazemian, Marc S. Halfon
    Pages 117-139
  9. Maria V. Sharakhova, Gleb N. Artemov, Vladimir A. Timoshevskiy, Igor V. Sharakhov
    Pages 177-194
  10. Hannes Schuler, Jacqueline A. Lopez, Meredith M. Doellman, Glen R. Hood, Scott P. Egan, Jeffrey L. Feder
    Pages 195-212
  11. Keshava Mysore, Limb K. Hapairai, Na Wei, Jacob S. Realey, Nicholas D. Scheel, David W. Severson et al.
    Pages 213-231
  12. Back Matter
    Pages 233-237

About this book


This volume focuses on the latest methods used to sequence, assemble, and analyze insect genomes. The collection of protocols in this book provides an introduction to the workflows and bioinformatics tools available for researchers. The chapters cover a range of useful topics such as determining genome size by flow cytometry; High Molecular Weight DNA extraction; improvements to a genome assembly provided by long-range sequencing approaches; assessments of orthology and single-copy genes at different phylogenetic levels; detecting regulatory regions with FAIRE, RAMPAGE, and computational analysis of cis-regulatory modules in insects; bioinformatics analysis of epigenetic modifications, high-throughput scanning of insect genomes (TEEseq) for the presence of endosymbionts, and leveraging genome sequence information to design RNAi strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Insect Genomics: Methods and Protocols is a valuable resource for graduate students, postdocs, and novice research scientists who are interested in learning more about this developing field.


Genome sequencing Developmental biology research Arthropods Bioinformatics tools Computational approaches Toxicology research

Editors and affiliations

  • Susan J. Brown
    • 1
  • Michael E. Pfrender
    • 2
  1. 1.Division of BiologyKansas State UniversityManhattanUSA
  2. 2.Department of Biological SciencesUniversity of Notre DameNotre DameUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8774-0
  • Online ISBN 978-1-4939-8775-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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