X-Chromosome Inactivation

Methods and Protocols

  • Takashi Sado

Part of the Methods in Molecular Biology book series (MIMB, volume 1861)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Asun Monfort, Giulio Di Minin, Anton Wutz
    Pages 1-18
  3. Ci Chu, Howard Y. Chang
    Pages 37-45
  4. Atsushi Fukuda, Akihiro Umezawa, Hidenori Akutsu
    Pages 47-53
  5. Osamu Masui, Edith Heard, Haruhiko Koseki
    Pages 67-72
  6. Emma R. Stewart, Dawn Coverley
    Pages 103-112
  7. Kris G. Alavattam, Hironori Abe, Akihiko Sakashita, Satoshi H. Namekawa
    Pages 113-129
  8. Aristea Magaraki, Agnese Loda, Joost Gribnau, Willy M. Baarends
    Pages 131-147
  9. Hirosuke Shiura, Yuka Sakata, Kuniya Abe, Takashi Sado
    Pages 161-176
  10. Marissa Cloutier, Clair Harris, Srimonta Gayen, Emily Maclary, Sundeep Kalantry
    Pages 177-203
  11. Wenxiu Ma, Giancarlo Bonora, Joel B. Berletch, Xinxian Deng, William S. Noble, Christine M. Disteche
    Pages 205-219
  12. Hisashi Miura, Rawin Poonperm, Saori Takahashi, Ichiro Hiratani
    Pages 221-245
  13. Back Matter
    Pages 247-251

About this book


This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture.


pluripotent stem cells trophoblast stem cells DNA methylation Xist RNA epigenetic

Editors and affiliations

  • Takashi Sado
    • 1
  1. 1.Department of Advanced Bioscience, Graduate School of AgricultureKindai UniversityNaraJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8765-8
  • Online ISBN 978-1-4939-8766-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals