Methods and Protocols

  • Rutilio Fratti

Part of the Methods in Molecular Biology book series (MIMB, volume 1860)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Biophysics and Computational Analysis

    1. Front Matter
      Pages 1-1
    2. Ryan Khounlo, Brenden J. D. Hawk, Yeon-Kyun Shin
      Pages 33-51
    3. Feng Li, Frederic Pincet
      Pages 71-93
    4. Lu Ma, Junyi Jiao, Yongli Zhang
      Pages 95-114
    5. Andrew E. Whitten, Russell J. Jarrott, Shu-Hong Hu, Anthony P. Duff, Gordon J. King, Jennifer L. Martin et al.
      Pages 115-144
  3. Biochemistry

    1. Front Matter
      Pages 161-161
    2. Yusuke Kishimoto, Takaaki Akaike, Hideshi Ihara
      Pages 163-173
    3. Kunrong Mei, Peng Yue, Wei Guo
      Pages 175-189
    4. Matthew L. Starr, Rutilio Fratti
      Pages 211-220
    5. Ekaterina Stroeva, Shyam S. Krishnakumar
      Pages 221-236
    6. Haijia Yu, Lauren Crisman, Michael H. B. Stowell, Jingshi Shen
      Pages 237-249
  4. Functional Assays

  5. Microscopy

    1. Front Matter
      Pages 333-333
    2. Yachong Hu, Zhiqi Tian, Jiajie Diao
      Pages 335-344
    3. Sevan Mattie, Tom Kazmirchuk, Jeannie Mui, Hojatollah Vali, Christopher Leonard Brett
      Pages 361-377
    4. Hao Xu, Bryan Stewart
      Pages 391-401
  6. Back Matter
    Pages 403-407

About this book


This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. The methods described in each chapter are described in detail so that novice as well as experienced researchers can explore the mechanisms of SNARE-mediated membrane fusion. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17, examining the structures and interactions of SNAREs, measuring the interactions of SNAREs, interactions of SNAREs, and post-translational modifications of SNAREs and how they affect function.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, SNAREs: Methods and Protocols aims to be a valuable tool for all investigators interested in the field.


Eukaryotic cells membrane trafficking post-translational modifications Reconstituted proteoliposomes Liposomes

Editors and affiliations

  • Rutilio Fratti
    • 1
  1. 1.Department of BiochemistryUniversity of Illinois Urbana-ChampaignUrbanaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2019
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8759-7
  • Online ISBN 978-1-4939-8760-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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