• Kathryn M. Murphy

Part of the Neuromethods book series (NM, volume 141)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Kathryn M. Murphy
      Pages 3-6
  3. History of Synaptosomes

    1. Front Matter
      Pages 7-7
    2. Peter R. Dunkley, Phillip J. Robinson
      Pages 27-53
  4. Making Synaptosomes

    1. Front Matter
      Pages 55-55
    2. Véronique De-Smedt-Peyrusse, Laetitia Darriet, Pierre Trifilieff, Etienne Herzog, Maria Florencia Angelo
      Pages 75-84
    3. Jing Xue, Annie Quan, Phillip J. Robinson
      Pages 85-105
  5. Studying Synaptic Energy and Metabolism Using Synaptosomes

    1. Front Matter
      Pages 107-107
    2. David G. Nicholls
      Pages 109-129
    3. Silvia Lores-Arnaiz, Georgina Rodríguez de Lores Arnaiz, Analía G. Karadayian, Juanita Bustamante
      Pages 131-151
    4. Sergei V. Baranov, Nicolas K. Khattar, Svitlana Yablonska, Oxana V. Baranova, Eric S. Kretz, Timothy M. Larkin et al.
      Pages 153-163
    5. Kasper Engholm-Keller, Nicolai Bache, Sushma R. Rao, Jesse R. Wark, Martin R. Larsen, Phillip J. Robinson et al.
      Pages 165-191
  6. Studying Synaptic Proteomics, Function, and Plasticity Using Synaptosomes

  7. Studying Development and Disease Using Synaptosomes

    1. Front Matter
      Pages 251-251
    2. Caitlin R. Siu, Simon P. Beshara, Justin L. Balsor, Steven J. Mancini, Kathryn M. Murphy
      Pages 269-286
    3. Nadeem Murtaza, Vickie Kwan, Biren Dave, Karun K. Singh
      Pages 287-296
    4. Nadia O. Postupna, Caitlin S. Latimer, C. Dirk Keene, Kathleen S. Montine, Thomas J. Montine, Martin Darvas
      Pages 297-310
  8. Back Matter
    Pages 311-312

About this book


This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses. After examining the history that led to making synaptosomes, the chapters delved into its important as a tool to explore synaptic connections. Some of the topics covered in this book are: synaptosome bioenergetics and glutamate release; affinity proteomics for interactome and phosphoproteome screening in synaptosomes; isolation of highly purified and functionally active synaptic mitochondria; and profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and thorough, Synaptosomes is a valuable resource for any researcher interested in learning more about this developing field.


synaptoneurosomes Cryoloading Protein synthesis oxidative stress gamma-aminobutyric

Editors and affiliations

  • Kathryn M. Murphy
    • 1
  1. 1.Department of Psychology, Neuroscience & BehaviorMcMaster UniversityHamiltonCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8738-2
  • Online ISBN 978-1-4939-8739-9
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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