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The Ubiquitin Proteasome System

Methods and Protocols

  • Thibault Mayor
  • Gary Kleiger

Part of the Methods in Molecular Biology book series (MIMB, volume 1844)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Methods to Uncover the Mechanisms of Action for Enzymes that Assemble or Disassemble Poly-Ubiquitin Chains

  3. Tools to Study Ubiquitylation

    1. Front Matter
      Pages 71-71
    2. Martin A. Michel, David Komander, Paul R. Elliott
      Pages 73-84
    3. Hazel F. O’Connor, Caleb D. Swaim, Larissa A. Canadeo, Jon M. Huibregtse
      Pages 85-100
    4. Yifat Geffen, Alon Appleboim, Richard G. Gardner, Tommer Ravid
      Pages 121-136
    5. Olga Levin-Kravets, Tal Keren-Kaplan, Ilan Attali, Itai Sharon, Neta Tanner, Dar Shapira et al.
      Pages 155-166
  4. Structural Approaches as Applied to Enzymes that Participate in the Ubiquitin Proteasome System

  5. Methods to Study 26S Proteasome Function

    1. Front Matter
      Pages 217-217
    2. Jeroen Roelofs, Anjana Suppahia, Kenrick A. Waite, Soyeon Park
      Pages 237-260
    3. Chueh-Ling Kuo, Galen Andrew Collins, Alfred L. Goldberg
      Pages 277-288
    4. Hyoung Tae Kim, Galen Andrew Collins, Alfred L. Goldberg
      Pages 289-308
    5. Jordan J. S. VerPlank, Alfred L. Goldberg
      Pages 309-319
    6. Amit Kumar Singh Gautam, Kirby Martinez-Fonts, Andreas Matouschek
      Pages 321-341
  6. Proteomic Methods to Study the Ubiquitin Proteasome System

  7. Back Matter
    Pages 411-413

About this book

Introduction

This volume explores numerous techniques used to study the ubiquitin proteasome system. The chapters in this book are organized into five parts and cover topics such as determining the mechanisms of action for E2s, E3s, and DUB enzymes; the latest advances to study the formation of poly-ubiquitin chains as well as their linkage types; the binding partners of proteins in the UPS; methods for structure determination by x-ray crystallography, cryo electron microscopy and SAXS; screening assays to select for degrons or modulators of E3s and DUBs; proteomics approaches in the ubiquitin field and methods to study 26S proteasome function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Thorough and authoritative, The Ubiquitin Proteasome System: Methods and Protocols is a valuable resource for both experienced and novice scientists who are interested in expanding their knowledge in this field.

Keywords

ubiquitination E2 enzymes poly-ubiquitin chains flow cytometry Cul4

Editors and affiliations

  • Thibault Mayor
    • 1
  • Gary Kleiger
    • 2
  1. 1.Department of Biochemistry and Molecular Biology, Michael Smith LaboratoriesUniversity of British ColumbiaVancouverCanada
  2. 2.Department of Chemistry and BiochemistryUniversity of Nevada Las VegasLas VegasUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8706-1
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8705-4
  • Online ISBN 978-1-4939-8706-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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