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Somatic Stem Cells

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Shree Ram Singh

    1. Basic Research Laboratory, Stem Cell Regulation and Animal Aging Section, National Cancer Institute, Frederick, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Pranela Rameshwar

    1. Department of Medicine-Hematology/Oncology, Rutgers New Jersey Medical School, Newark, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1842)

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Table of contents (20 protocols)

  1. Stem Cell Study in Model Organisms and Human

    1. Beating Heart Cells from Hair-Follicle-Associated Pluripotent (HAP) Stem Cells

      • Robert M. Hoffman, Yasuyuki Amoh
      Pages 241-254

    2. Generation of FLIP and FLIP-FlpE Targeting Vectors for Biallelic Conditional and Reversible Gene Knockouts in Mouse and Human Cells

      • Bon-Kyoung Koo
      Pages 255-264

    3. Analytical Platforms and Techniques to Study Stem Cell Metabolism

      • Christine Tang, Kevin Chen, Aleksandar Bajic, William T. Choi, Dodge L. Baluya, Mirjana Maletic-Savatic
      Pages 265-281

  2. Back Matter

    Pages 283-284
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Keywords

About this book

This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.



Editors and Affiliations

  • Basic Research Laboratory, Stem Cell Regulation and Animal Aging Section, National Cancer Institute, Frederick, USA

    Shree Ram Singh

  • Department of Medicine-Hematology/Oncology, Rutgers New Jersey Medical School, Newark, USA

    Pranela Rameshwar

Bibliographic Information

  • Book Title: Somatic Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Shree Ram Singh, Pranela Rameshwar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8697-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8696-5Published: 09 September 2018

  • Softcover ISBN: 978-1-4939-9369-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8697-2Published: 08 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 284

  • Number of Illustrations: 6 b/w illustrations, 52 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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