© 2018

Somatic Stem Cells

Methods and Protocols

  • Shree Ram Singh
  • Pranela Rameshwar


  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1842)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Pranela Rameshwar, Caitlyn A. Moore, Niloy N. Shah, Caroline P. Smith
      Pages 3-27
  3. Stem Cell Study in Model Organisms and Human

    1. Front Matter
      Pages 29-29
    2. Mayra Garcia, Lihong Weng, Xingbin Hu, Ching-Cheng Chen
      Pages 43-54
    3. Bijender Kumar, Srideshikan Sargur Madabushi
      Pages 55-68
    4. Xiaoyang Wang, Yizhan Xing, Yuhong Li
      Pages 69-80
    5. Peter Conaty, Lauren S. Sherman, Yahaira Naaldijk, Henning Ulrich, Alexandra Stolzing, Pranela Rameshwar
      Pages 81-91
    6. Caitlyn A. Moore, Niloy N. Shah, Caroline P. Smith, Pranela Rameshwar
      Pages 93-103
    7. Carlos Antônio do Nascimento Santos, Radovan Borojevic, Luiz Eurico Nasciutti, Christina M. Maedatakiya
      Pages 105-121
    8. Nathan Pinto, Beyoncé Carrington, Catharine Dietrich, Rachit Sinha, Cristopher Aguilar, Tiffany Chen et al.
      Pages 123-137
    9. Leigh A. Samsa, Ian A. Williamson, Scott T. Magness
      Pages 139-166
    10. Ivan Bertoncello, Gianni Carraro, Jonathan L. McQualter
      Pages 167-181
    11. Santosh Kumar Yadav, Paras Kumar Mishra
      Pages 183-191
    12. Guangpeng Liu, Xi Chen
      Pages 193-201
    13. Lauren S. Sherman, Alexandra Condé-Green, Vasanth S. Kotamarti, Edward S. Lee, Pranela Rameshwar
      Pages 203-206
    14. Qianping Guo, Pinghui Zhou, Bin Li
      Pages 207-216
    15. Jianying Zhang, James H.-C. Wang
      Pages 217-228

About this book


This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.


Zebrafish Progenitor Cells Clinical Translation miRNA murine hearts regenerative medicines stem cells

Editors and affiliations

  • Shree Ram Singh
    • 1
  • Pranela Rameshwar
    • 2
  1. 1.Basic Research LaboratoryStem Cell Regulation and Animal Aging Section, National Cancer InstituteFrederickUSA
  2. 2.Department of Medicine-Hematology/OncologyRutgers New Jersey Medical SchoolNewarkUSA

Bibliographic information

  • Book Title Somatic Stem Cells
  • Book Subtitle Methods and Protocols
  • Editors Shree Ram Singh
    Pranela Rameshwar
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-8696-5
  • Softcover ISBN 978-1-4939-9369-7
  • eBook ISBN 978-1-4939-8697-2
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 2
  • Number of Pages XI, 284
  • Number of Illustrations 6 b/w illustrations, 52 illustrations in colour
  • Topics Cell Biology
  • Buy this book on publisher's site
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