© 2018

Microbial Proteomics

Methods and Protocols

  • Dörte Becher
  • Includes cutting-edge methods and protocols for the study of microbial proteomics

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 1841)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Global Sample Preparation and Protein Enrichment

    1. Front Matter
      Pages 1-1
  3. Subcellular Fractionation

  4. Protein Quantification

    1. Front Matter
      Pages 77-77
    2. Martin Moche, Dirk Albrecht, Reiner Westermeier, Knut Büttner
      Pages 79-93
    3. Susanne Engelmann, Stephan Fuchs
      Pages 113-130
    4. Florian-Alexander Herbst, Nico Jehmlich, Martin von Bergen, Frank Schmidt
      Pages 163-174
    5. Claudia Lindemann, Nikolas Thomanek, Katja Kuhlmann, Helmut E. Meyer, Katrin Marcus, Franz Narberhaus
      Pages 189-206
    6. Henrike Pförtner, Maren Depke, Kristin Surmann, Frank Schmidt, Uwe Völker
      Pages 207-228
  5. Analysis of Post Translational Protein Modifications

    1. Front Matter
      Pages 229-229

About this book


This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond.


Subcellular fractionation Protein quantification Metaproteomics Microbes Proteome analysis Post-translational protein modifications

Editors and affiliations

  • Dörte Becher
    • 1
  1. 1.Department of Microbial Proteomics, Institute for MicrobiologyUniversity GreifswaldGreifswaldGermany

Bibliographic information

  • Book Title Microbial Proteomics
  • Book Subtitle Methods and Protocols
  • Editors Dörte Becher
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-8693-4
  • Softcover ISBN 978-1-4939-9368-0
  • eBook ISBN 978-1-4939-8695-8
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XV, 344
  • Number of Illustrations 24 b/w illustrations, 26 illustrations in colour
  • Topics Protein Science
  • Buy this book on publisher's site
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