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Microbial Proteomics

Methods and Protocols

  • Dörte Becher

Part of the Methods in Molecular Biology book series (MIMB, volume 1841)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Global Sample Preparation and Protein Enrichment

    1. Front Matter
      Pages 1-1
  3. Subcellular Fractionation

  4. Protein Quantification

    1. Front Matter
      Pages 77-77
    2. Martin Moche, Dirk Albrecht, Reiner Westermeier, Knut Büttner
      Pages 79-93
    3. Susanne Engelmann, Stephan Fuchs
      Pages 113-130
    4. Florian-Alexander Herbst, Nico Jehmlich, Martin von Bergen, Frank Schmidt
      Pages 163-174
    5. Claudia Lindemann, Nikolas Thomanek, Katja Kuhlmann, Helmut E. Meyer, Katrin Marcus, Franz Narberhaus
      Pages 189-206
    6. Henrike Pförtner, Maren Depke, Kristin Surmann, Frank Schmidt, Uwe Völker
      Pages 207-228
  5. Analysis of Post Translational Protein Modifications

    1. Front Matter
      Pages 229-229
    2. Boumediene Soufi, Christoph Täumer, Maja Semanjski, Boris Macek
      Pages 231-247
    3. Angel Manteca, Beatriz Rioseras, Adelina Rogowska-Wrzesinska, Ole N. Jensen
      Pages 249-260
    4. Martina Rossius, Falko Hochgräfe, Haike Antelmann
      Pages 261-275
  6. Metaproteomics

  7. Back Matter
    Pages 335-344

About this book

Introduction

This detailed volume explores state-of-the-art methods for the identification, quantification, and characterization of microbial proteins. Split into five parts, the content addresses global sample preparation and protein enrichment, subcellular fractionation, protein quantification, analysis of post-translational protein modifications, as well as metaproteomics, a relatively new branch of microbial proteomics that investigates the proteins of all microbes comprising an environmental consortium. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microbial Proteomics: Methods and Protocols serves as a valuable and stimulating source for all beginners and advanced researchers in the field of microbial proteomics and beyond.

Keywords

Subcellular fractionation Protein quantification Metaproteomics Microbes Proteome analysis Post-translational protein modifications

Editors and affiliations

  • Dörte Becher
    • 1
  1. 1.Department of Microbial Proteomics, Institute for MicrobiologyUniversity GreifswaldGreifswaldGermany

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8695-8
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8693-4
  • Online ISBN 978-1-4939-8695-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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