The LINC Complex

Methods and Protocols

  • Gregg G. Gundersen
  • Howard J. Worman

Part of the Methods in Molecular Biology book series (MIMB, volume 1840)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Structural and Biochemical Analysis of LINC Complexes

    1. Front Matter
      Pages 1-1
    2. Marie-Christin Spindler, Frederik Helmprobst, Christian Stigloher, Ricardo Benavente
      Pages 3-15
    3. Victor E. Cruz, Thomas U. Schwartz
      Pages 17-23
    4. Susumu Antoku, Gregg G. Gundersen
      Pages 35-43
  3. Mechanical Aspects of the LINC Complex

    1. Front Matter
      Pages 57-57
    2. Paul T. Arsenovic, Daniel E. Conway
      Pages 59-71
    3. Nejma Belaadi, Angélique Millon-Frémillon, Julien Aureille, Christophe Guilluy
      Pages 73-80
    4. Vincent J. Tocco, Srujana Neelam, Qiao Zhang, Richard B. Dickinson, Tanmay P. Lele
      Pages 81-90
  4. Analysis of the LINC Complex in Model Systems and Development

    1. Front Matter
      Pages 119-119
    2. Jared Hennen, Isaac Angert, Kwang-Ho Hur, G. W. Gant Luxton, Joachim D. Mueller
      Pages 121-135
    3. Heidi N. Fridolfsson, Leslie A. Herrera, James N. Brandt, Natalie E. Cain, Greg J. Hermann, Daniel A. Starr
      Pages 163-180
    4. Alexander L. Auld, Mary Ann Collins, Torrey R. Mandigo, Eric S. Folker
      Pages 181-203
    5. Axel Poulet, Xiao Zhou, Kentaro Tamura, Iris Meier, Christophe Tatout, Katja Graumann et al.
      Pages 205-219
  5. LINC Complex in Mammalian Tissue, Organs and Disease

    1. Front Matter
      Pages 249-249
    2. Matthew J. Stroud, Xi Fang, Jennifer Veevers, Ju Chen
      Pages 251-281
    3. Iakowos Karakesisoglou, Carmen Mroß, Angelika A. Noegel
      Pages 295-306
    4. Ayaka Matsumoto, Nariaki Matsuura, Miki Hieda
      Pages 307-319
  6. Back Matter
    Pages 337-338

About this book


This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in mammalian tissue, organs, and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Nucleoskeleton Cytoskeleton SUN domain KASH domain tissue-selective expression

Editors and affiliations

  • Gregg G. Gundersen
    • 1
  • Howard J. Worman
    • 2
  1. 1.Department of Pathology and Cell BiologyColumbia UniversityNew YorkUSA
  2. 2.Department of Medicine and Department of Pathology and Cell BiologyColumbia UniversityNew YorkUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8690-3
  • Online ISBN 978-1-4939-8691-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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