© 2018

Histone Variants

Methods and Protocols

  • Guillermo A. Orsi
  • Geneviève Almouzni

Part of the Methods in Molecular Biology book series (MIMB, volume 1832)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Biochemistry

    1. Front Matter
      Pages 1-1
    2. Tomoya Kujirai, Yasuhiro Arimura, Risa Fujita, Naoki Horikoshi, Shinichi Machida, Hitoshi Kurumizaka
      Pages 3-20
    3. Francisco Saavedra, Sebastián Marty-Lombardi, Alejandra Loyola
      Pages 21-49
    4. Moritz Carl Völker-Albert, Andreas Schmidt, Teresa K. Barth, Ignasi Forne, Axel Imhof
      Pages 61-74
  3. Epigenomics

    1. Front Matter
      Pages 75-75
    2. Alicia Alonso, Emily Bernstein, Dan Hasson
      Pages 77-104
    3. Julia Truch, Jelena Telenius, Douglas R. Higgs, Richard J. Gibbons
      Pages 105-130
    4. Wojciech Siwek, Mariluz Gómez-Rodríguez, Daniel Sobral, Ivan R. Corrêa Jr, Lars E. T. Jansen
      Pages 131-158
    5. Srinivas Ramachandran, Steven Henikoff
      Pages 159-168
    6. Tatiana A. Soboleva, David J. Tremethick
      Pages 169-184
    7. Jakub Mieczkowski, Michael Y. Tolstorukov
      Pages 185-203
  4. Microscopy and Cell Biology

    1. Front Matter
      Pages 205-205
    2. Júlia Torné, Guillermo A. Orsi, Dominique Ray-Gallet, Geneviève Almouzni
      Pages 207-221
    3. Juliette Dabin, Anna Fortuny, Sandra Piquet, Sophie E. Polo
      Pages 243-253
    4. Justin W. C. Leung, Lara E. Emery, Kyle M. Miller
      Pages 255-269
  5. Evolution, Development, and Diseases

    1. Front Matter
      Pages 271-271
    2. Antoine Molaro, Ines A. Drinnenberg
      Pages 273-291

About this book


This volume compiles methodologies used for detailed studies of histone variants, from their basic properties to their functional roles in chromatin and as vectors of epigenetic information. Its four sections cover experimental approaches to probe the biochemistry of histone variants and variant nucleosomes; their dynamics throughout the cell cycle and at specific genomic locations; their functional impact on chromosome organization and genome stability; and their importance in development, disease and evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, illustrations on the expected results, and tips on troubleshooting and avoiding known pitfalls. This anthology brings together contributions from many of the most highly recognized investigators in this community.

Cutting-edge and thorough, Histone Variants: Methods and Protocols is a valuable resource for anyone interested in exploring the experimental and collaborative possibilities in this evolving field.


chromatin integrity nucleosomes histone chaperones cellular functions epigenomics

Editors and affiliations

  • Guillermo A. Orsi
    • 1
  • Geneviève Almouzni
    • 2
  1. 1.Institut CuriePSL Research University CNRS, UMR3664, Equipe Labellisée Ligue contre le CancerParisFrance
  2. 2.Institut CuriePSL Research University CNRS, UMR3664, Equipe Labellisée Ligue contre le CancerParisFrance

Bibliographic information

  • Book Title Histone Variants
  • Book Subtitle Methods and Protocols
  • Editors Guillermo A. Orsi
    Geneviève Almouzni
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-8662-0
  • Softcover ISBN 978-1-4939-9358-1
  • eBook ISBN 978-1-4939-8663-7
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XII, 359
  • Number of Illustrations 19 b/w illustrations, 43 illustrations in colour
  • Topics Protein Science
  • Buy this book on publisher's site
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