Plant Transcription Factors

Methods and Protocols

  • Nobutoshi Yamaguchi

Part of the Methods in Molecular Biology book series (MIMB, volume 1830)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Review and Overview Chapters

  3. Preparation of Plant Materials

    1. Front Matter
      Pages 59-59
    2. Pooja Aggarwal, Krishna Reddy Challa, Monalisha Rath, Preethi Sunkara, Utpal Nath
      Pages 61-79
    3. Beibei Zheng, Bennett Thomson, Frank Wellmer
      Pages 81-92
    4. Ze Hong Lee, Nobutoshi Yamaguchi, Toshiro Ito
      Pages 93-108
    5. Tatyana Radoeva, Shunsuke Saiga, Dolf Weijers
      Pages 127-139
    6. Kyohei Uemoto, Takashi Araki, Motomu Endo
      Pages 141-148
  4. Detection of Expression Levels

    1. Front Matter
      Pages 161-161
    2. María Katherine Mejia-Guerra, Wei Li, Andrea I. Doseff, Erich Grotewold
      Pages 239-256
  5. Interaction Tests

  6. Chromation Analyses

  7. Back Matter
    Pages 395-396

About this book


This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Transcription Factors: Methods and Protocols aims to answer a wide range of questions related to transcription factors commonly raised by plant biologists.


Plant genetics Gene expression Chromatin analysis Plant biology CRISPR/CAS9 Plant development

Editors and affiliations

  • Nobutoshi Yamaguchi
    • 1
  1. 1.Plant Stem Cell Regulation and Floral Patterning LaboratoryNara Institute of Science and TechnologyIkomaJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8656-9
  • Online ISBN 978-1-4939-8657-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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