Antibody Engineering

Methods and Protocols

  • Damien Nevoltris
  • Patrick Chames

Part of the Methods in Molecular Biology book series (MIMB, volume 1827)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. In Silico Methods

    1. Front Matter
      Pages 1-1
    2. Vinodh B. Kurella, Reddy Gali
      Pages 3-14
    3. Daisuke Kuroda, Kouhei Tsumoto
      Pages 15-34
    4. Marie-Paule Lefranc, François Ehrenmann, Sofia Kossida, Véronique Giudicelli, Patrice Duroux
      Pages 35-69
  3. Generation of Diversity

    1. Front Matter
      Pages 71-71
    2. Michela Pasello, Alessandra Mallano, Michela Flego, Silvia Zamboni, Anna Maria Giudice, Katia Scotlandi
      Pages 73-91
    3. Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau
      Pages 93-108
    4. Doreen Könning, Steffen Hinz, Julius Grzeschik, Christian Schröter, Simon Krah, Stefan Zielonka et al.
      Pages 109-127
    5. Vladimir Bobkov, Bas van der Woning, Hans de Haard
      Pages 129-144
    6. Simon Krah, Julius Grzeschik, Simon Rosowski, Ramona Gaa, Iris Willenbuecher, Deniz Demir et al.
      Pages 145-161
  4. Selections of Lead Candidate

    1. Front Matter
      Pages 163-163
    2. Magali Colazet, Patrick Chames
      Pages 165-178
    3. Martina L. Jones, Stephen M. Mahler, Sumukh Kumble
      Pages 179-195
    4. Mahdi Zeraati, Marcel E. Dinger, Daniel Christ
      Pages 197-209
    5. Nathalie Scholler
      Pages 211-233
    6. Naoto Nemoto, Shigefumi Kumachi, Hidenao Arai
      Pages 269-285
    7. Peter Schofield, Rodrigo Vazquez-Lombardi, Mahmoud Abdelatti, Damien Nevoltris, Christopher C. Goodnow, Daniel Christ et al.
      Pages 287-309
  5. Production of Recombinant Antibodies

    1. Front Matter
      Pages 311-311
    2. Rodrigo Vazquez-Lombardi, Damien Nevoltris, Romain Rouet, Daniel Christ
      Pages 313-334
  6. Fc Engineering

    1. Front Matter
      Pages 365-365
    2. Christian Kellner, Stefanie Derer, Katja Klausz, Sophia Rosskopf, Tim Wirt, Thies Rösner et al.
      Pages 381-397
    3. Abhishek Saxena, Bingxin Bai, Shin-Chen Hou, Lianlian Jiang, Tianlei Ying, Shane Miersch et al.
      Pages 399-417
  7. Characterization, Optimization, and Innovative Formats

    1. Front Matter
      Pages 419-419
    2. Stephen Hearty, Paul Leonard, Hui Ma, Richard O’Kennedy
      Pages 421-455
    3. Peter M. Bowers, William J. Boyle, Robert Damoiseaux
      Pages 479-489
    4. Sandrine Moutel, Clément Nizak, Franck Perez
      Pages 491-503
    5. Maxine Crauwels, Sam Massa, Charlotte Martin, Cecilia Betti, Steven Ballet, Nick Devoogdt et al.
      Pages 505-540
  8. Back Matter
    Pages 541-543

About this book


This detailed new edition provides complete and easy access to a variety of antibody engineering techniques.  The volume explores topics such as the generation of native, synthetic, or immune antibody libraries, the selection of lead candidates via the different powerful and innovative display technologies, Fc engineering, as well as their production, characterization, and optimization of antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Antibody Engineering: Methods and Protocols, Third Edition presents the reader with an extensive toolbox to create the powerful molecules of tomorrow.


Antibody-based therapeutics Antibody gene libraries Recombinant antibodies Fc engineering Monoclonal antibodies Therapeutic drugs Therapeutic antibodies

Editors and affiliations

  • Damien Nevoltris
    • 1
  • Patrick Chames
    • 2
  1. 1.Immunology DivisionGarvan Institute of Medical ResearchDarlinghurstAustralia
  2. 2.Institut Paoli-CalmettesCRCM, Aix Marseille University, CNRS, INSERMMarseilleFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8647-7
  • Online ISBN 978-1-4939-8648-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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