Functional Genomics in Medicago truncatula

Methods and Protocols

  • Luis A. Cañas
  • José Pío Beltrán

Part of the Methods in Molecular Biology book series (MIMB, volume 1822)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Luis A. Cañas, José Pío Beltrán
    Pages 11-37
  3. David Burks, Rajeev Azad, Jiangqi Wen, Rebecca Dickstein
    Pages 39-59
  4. Myriam Sánchez, Christine Le Signor, Gregoire Aubert, Brigitte Darchy, Karine Gallardo, Richard D. Thompson
    Pages 71-82
  5. Francesco Panara, Ornella Calderini, Andrea Porceddu
    Pages 83-105
  6. Hee-Kyung Lee, Kirankumar S. Mysore, Jiangqi Wen
    Pages 107-114
  7. Joanna Serwatowska, Ole Søgaard Lund, Ida Elisabeth Johansen
    Pages 115-122
  8. Hélène Proust, Jérémie Bazin, Céline Sorin, Caroline Hartmann, Martin Crespi, Christine Lelandais-Brière
    Pages 123-132
  9. Concepción Gómez-Mena, Edelín M. Roque
    Pages 133-144
  10. Olga Kulikova, Carolien Franken, Ton Bisseling
    Pages 145-159
  11. Christine Le Signor, Vanessa Vernoud, Mélanie Noguero, Karine Gallardo, Richard D. Thompson
    Pages 175-195
  12. Hélène Proust, Caroline Hartmann, Martin Crespi, Christine Lelandais-Brière
    Pages 205-239
  13. Fathi Berrabah, El Hosseyn Ait Salem, Marie Garmier, Pascal Ratet
    Pages 241-260
  14. James L. Weller, Richard C. Macknight
    Pages 261-271
  15. Edelín Roque, Concepción Gómez-Mena, Cristina Ferrándiz, José Pío Beltrán, Luis A. Cañas
    Pages 273-290
  16. Chenggang Liu, Chan Man Ha, Richard A. Dixon
    Pages 315-337
  17. Back Matter
    Pages 339-343

About this book


This volume discusses popular methods to achieve different types of mutagenesis and forward/reverse genetics in Medicago truncatula. Several studies on genetic control of developmental and metabolic processes in this model legume are also described. The chapters in this book cover topics such as Targeting Induced Local Lesions IN Genomes (TILLING), Fast Neutron Bombardment (FNB), Tnt1 insertional mutagenesis, Virus-Induced Gene Silencing (VIGS), stable inactivation of microRNAs in roots, gene editing by CRISPR-Cas9, etc. This book also contains reviews on the specific use of these techniques in functional studies on the genetic control of seed, leaf, root, nodule, floral and fruit development in M. truncatula. Written for the highly successful Methods in Molecular Biology series format, chapters contain the kind of detailed description and implementation advice needed to promote success in the lab.

Cutting-edge and thorough, Functional Genomics in Medicago truncatula: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.


CRISPR-Cas9 TILLING genome sequencing FNB activation tagging

Editors and affiliations

  • Luis A. Cañas
    • 1
  • José Pío Beltrán
    • 2
  1. 1.CSIC-UPVInstitute for Plant Cell and Molecular Biology (IBMCP)ValenciaSpain
  2. 2.CSIC-UPVInstitute for Plant Cell and Molecular Biology (IBMCP)ValenciaSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8632-3
  • Online ISBN 978-1-4939-8633-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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