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Epithelial Cell Culture

Methods and Protocols

  • Mario Baratta

Part of the Methods in Molecular Biology book series (MIMB, volume 1817)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Sarah J. Morgan, Susanne Neumann, Marvin C. Gershengorn
    Pages 1-7
  3. Danielle Wu, Patricia Chapela, Mary C. Farach-Carson
    Pages 19-32
  4. Macarena P. Quintana-Hayashi, Sara K. Lindén
    Pages 41-46
  5. Kamila Maliszewska-Olejniczak, Klaudia K. Brodaczewska, Zofia F. Bielecka, Anna M. Czarnecka
    Pages 47-66
  6. Angelo Canciello, Luana Greco, Valentina Russo, Barbara Barboni
    Pages 67-78
  7. Bushra T. Mohammed, F. Xavier Donadeu
    Pages 79-87
  8. Massimo Faustini, Giulio Curone, Maria L. Torre, Daniele Vigo
    Pages 89-93
  9. Giovanni Giuseppe Leoni, Salvatore Naitana
    Pages 95-106
  10. Inês Pereira, Anna Lechanteur, Bruno Sarmento
    Pages 107-113
  11. Seung Bum Lee, Sung-Hoon Han, Sunhoo Park
    Pages 123-135
  12. Eugenio Martignani, Paolo Accornero, Silvia Miretti, Mario Baratta
    Pages 137-144
  13. Emma Teal, Nina Bertaux-Skeirik, Jayati Chakrabarti, Loryn Holokai, Yana Zavros
    Pages 145-155
  14. Jayati Chakrabarti, Loryn Holokai, LiJyun Syu, Nina Steele, Julie Chang, Andrzej Dlugosz et al.
    Pages 157-168
  15. Paolo Accornero, Eugenio Martignani, Silvia Miretti, Mario Baratta
    Pages 169-183
  16. Matheus O. Costa, Janet E. Hill, Michael K. Dame, John C. S. Harding
    Pages 185-195
  17. Back Matter
    Pages 209-214

About this book

Introduction

This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and  two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test  cell activities. Further,  The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.

Keywords

cancer mammary cell lines transcriptome Pig blastocyst culture Pancreatic bio-Scaffold cancer stem cell

Editors and affiliations

  • Mario Baratta
    • 1
  1. 1.Department of Veterinary ScienceUniversity of TurinTurinItaly

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-8600-2
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8599-9
  • Online ISBN 978-1-4939-8600-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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