ADP-ribosylation and NAD+ Utilizing Enzymes

Methods and Protocols

  • Paul Chang

Part of the Methods in Molecular Biology book series (MIMB, volume 1813)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Myron K. Jacobson, Elaine L. Jacobson
      Pages 3-8
  3. ADP-Ribose Binding Domains as Tools

    1. Front Matter
      Pages 9-9
    2. Giovanna Grimaldi, Giuliana Catara, Carmen Valente, Daniela Corda
      Pages 25-40
    3. Mareike Bütepage, Sarah Krieg, Laura Eckei, Jinyu Li, Giulia Rossetti, Patricia Verheugd et al.
      Pages 41-63
  4. Enzymatic Assays of NAD+ Utilizing Enzymes

    1. Front Matter
      Pages 75-75
    2. Michael B. Schultz, Yuancheng Lu, Nady Braidy, David A. Sinclair
      Pages 77-90
    3. Anna-Lena Kolb, Duen-Wei Hsu, Ana B. A. Wallis, Seiji Ura, Alina Rakhimova, Catherine J. Pears et al.
      Pages 125-148
    4. Stephan Menzel, Sahil Adriouch, Peter Bannas, Friedrich Haag, Friedrich Koch-Nolte
      Pages 167-186
    5. Jeannette Abplanalp, Ann-Katrin Hopp, Michael O. Hottiger
      Pages 205-213
    6. Melanija Posavec Marjanovic´, Gytis Jankevicius, Ivan Ahel
      Pages 215-223
    7. Jun Young Hong, Xiaoyu Zhang, Hening Lin
      Pages 225-234
  5. Small Molecule Screening Assays of NAD+ Utilizing Enzymes

    1. Front Matter
      Pages 235-235
    2. Teemu Haikarainen, Sudarshan Murthy, Mirko M. Maksimainen, Lari Lehtiö
      Pages 237-244
    3. Ilsa T. Kirby, Rory K. Morgan, Michael S. Cohen
      Pages 245-252
  6. Mass Spectrometry Techniques for Detection of Mono-ADP-Ribosylation

    1. Front Matter
      Pages 253-253
    2. Robert Lyle McPherson, Shao-En Ong, Anthony K. L. Leung
      Pages 271-283
  7. Mono-ADP-Ribose and Disease

    1. Front Matter
      Pages 285-285
    2. Chen Chen, Joseph T. Barbieri
      Pages 287-295
    3. Rachy Abraham, Robert Lyle McPherson, Easwaran Sreekumar, Anthony K. L. Leung, Diane E. Griffin
      Pages 297-316
    4. Björn Rissiek, Marco Lukowiak, Friedrich Haag, Tim Magnus, Friedrich Koch-Nolte
      Pages 317-326
    5. Florian J. Bock, Paul Chang
      Pages 327-341
  8. NAD+ Analogue Approaches and Chemical Synthesis

    1. Front Matter
      Pages 343-343
    2. Hans A. V. Kistemaker, Jim Voorneveld, Dmitri V. Filippov
      Pages 345-369
  9. NAD+ Sensors

    1. Front Matter
      Pages 389-389
    2. Michael S. Cohen, Melissa L. Stewart, Richard H. Goodman, Xiaolu A. Cambronne
      Pages 391-414
  10. Back Matter
    Pages 415-417

About this book


This volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols is a valuable resource for anyone interested in this developing and expanding field.


MonoPARPs Sirtuins CD38/ ARTc enzyme assays PARP

Editors and affiliations

  • Paul Chang
    • 1
  1. 1.Department of Biology, Koch Institute for Integrative Cancer ResearchMassachusetts Institute of TechnologyCambridgeUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8587-6
  • Online ISBN 978-1-4939-8588-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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