Handbook of ELISPOT

Methods and Protocols

  • Alexander E. Kalyuzhny

Part of the Methods in Molecular Biology book series (MIMB, volume 1808)

Table of contents

  1. Front Matter
    Pages i-x
  2. Jodi Hagen, Alexander E. Kalyuzhny
    Pages 31-41
  3. Sergey S. Zadorozhny, Nikolai N. Martynov
    Pages 43-50
  4. Jodi Hanson, Diana R. Roen, Paul V. Lehmann
    Pages 51-62
  5. Peter Jahnmatz, Niklas Ahlborg
    Pages 63-72
  6. Diana R. Roen, Jodi Hanson, Paul V. Lehmann
    Pages 73-83
  7. Zoltán Megyesi, Paul V. Lehmann, Alexey Y. Karulin
    Pages 115-131
  8. Alison J. Johnson, Steven C. Kennedy, Tony W. Ng, Steven A. Porcelli
    Pages 143-150
  9. Chandima Jeewandara, Graham S. Ogg, Gathsaurie Neelika Malavige
    Pages 165-171
  10. Achala Indika Kamaladasa, Gathsaurie Neelika Malavige
    Pages 181-186
  11. Bikash Sahay, Alek M. Aranyos, Andrew McAvoy, Janet K. Yamamoto
    Pages 197-219
  12. Iana H. Haralambieva, Inna G. Ovsyannikova, Richard B. Kennedy, Gregory A. Poland
    Pages 221-236
  13. J. C. Neubauer, I. Sébastien, A. Germann, H. von Briesen, H. Zimmermann
    Pages 237-247
  14. Back Matter
    Pages 249-251

About this book


This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.


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Editors and affiliations

  • Alexander E. Kalyuzhny
    • 1
  1. 1.Bio-Techne, Inc.MinneapolisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-8566-1
  • Online ISBN 978-1-4939-8567-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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