Type 2 Immunity

Methods and Protocols

  • R. Lee Reinhardt

Part of the Methods in Molecular Biology book series (MIMB, volume 1799)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. J. Morgan Knight, Evan Li, Hui-Ying Tung, Cameron Landers, Jake Wheeler, Farrah Kheradmand et al.
    Pages 1-9
  3. Nancy J. Reyes, Rose Mathew, Daniel R. Saban
    Pages 49-57
  4. Hideki Nakano, Keiko Nakano, Donald N. Cook
    Pages 59-69
  5. Hae Woong Choi, Soman N. Abraham
    Pages 71-80
  6. Heather L. Caslin, Marcela T. Taruselli, Anuya Paranjape, Kasalina Kiwanuka, Tamara Haque, Alena P. Chumanevich et al.
    Pages 81-92
  7. Jesse Charles Nussbaum, Jorge Felipe Ortiz-Carpena
    Pages 93-107
  8. Wanjiang Zeng, Rebecca L. O’Brien, Willi K. Born, Yafei Huang
    Pages 135-151
  9. Véronique Schulten, Alessandro Sette
    Pages 153-163
  10. Manon Vanheerswynghels, Wendy Toussaint, Martijn Schuijs, Leen Vanhoutte, Nigel Killeen, Hamida Hammad et al.
    Pages 183-210
  11. Mark Dell’Aringa, R. Lee Reinhardt
    Pages 211-223
  12. Mark Dell’Aringa, R. Lee Reinhardt, Rachel S. Friedman, Jordan Jacobelli
    Pages 225-235
  13. Miranda R. Lyons-Cohen, Hideki Nakano, Seddon Y. Thomas, Donald N. Cook
    Pages 237-246
  14. Zhiyong Yang, James B. Jung, Christopher D. C. Allen
    Pages 247-264
  15. Carine Bouffi, Artem Barski, Patricia C. Fulkerson
    Pages 265-274
  16. Sandy Lisette Rosales, Shu Liang, Isaac Engel, Benjamin Joachim Schmiedel, Mitchell Kronenberg, Pandurangan Vijayanand et al.
    Pages 275-302
  17. Diana Youhanna Jankeel, Justin Cayford, Benjamin Joachim Schmiedel, Pandurangan Vijayanand, Grégory Seumois
    Pages 303-326
  18. Laura D. Harmacek, Preeyam Patel, Rachel Woolaver, R. Lee Reinhardt, Brian P. O’Connor
    Pages 327-340
  19. Marlys S. Fassett, Heather H. Pua, Laura J. Simpson, David F. Steiner, K. Mark Ansel
    Pages 341-351
  20. Mei San Tang, Rowann Bowcutt, P’ng Loke
    Pages 359-370
  21. Sandy Lisette Rosales, Shu Liang, Isaac Engel, Benjamin Joachim Schmiedel, Mitchell Kronenberg, Pandurangan Vijayanand et al.
    Pages C3-C3
  22. Back Matter
    Pages 433-434

About this book


This book provides researchers the opportunity to investigate type-2-associated diseases in their laboratories. Beginning with chapters describing various models of type-2 immunity, the volume then continues by detailing cellular protocols designed to identify, characterize, and assess the function of key adaptive and innate immune cells involved in type-2 inflammation; approaches to isolate and evaluate specific cellular subsets at the genetic, epigenetic, and molecular level; protocols to assess type-2 immunity and its relationship to organismal and metabolic systems (ex. Microbiome). This book concludes with a section that explores the use of primary human cells in evaluating relevance to the clinic. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Vital and authoritative, Type 2 Immunity: Methods and Protocols aims to provide a broad network of methods that can be used to develop a hypothesis and investigate its potential from bench to beside. 


T cell Basophils Mast cells Eosinophils lymphoid cells

Editors and affiliations

  • R. Lee Reinhardt
    • 1
  1. 1.Department of Biomedical ResearchNational Jewish HealthDenverUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7895-3
  • Online ISBN 978-1-4939-7896-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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