Two-Hybrid Systems

Methods and Protocols

  • Luis Oñate-Sánchez

Part of the Methods in Molecular Biology book series (MIMB, volume 1794)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Soon Gang Choi, Aaron Richardson, Luke Lambourne, David E. Hill, Marc Vidal
    Pages 1-14
  3. Yeast Hybrid Systems to Study Protein, Nucleic Acid, and Ligand Interactions

    1. Front Matter
      Pages 15-15
    2. Jitender Mehla, J. Harry Caufield, Peter Uetz
      Pages 17-28
    3. Lisa Yasmin Asseck, Christopher Grefen
      Pages 49-60
    4. Macy G. Olson, Megan Goldammer, Emilie Gauliard, Daniel Ladant, Scot P. Ouellette
      Pages 75-96
    5. Shaowei Dong, Nicholas J. Provart
      Pages 97-117
    6. Jared A. Sewell, Juan I. Fuxman Bass
      Pages 119-130
    7. Rocío Sánchez-Montesino, Luis Oñate-Sánchez
      Pages 131-149
    8. Xiaoyu Ji, Liuqiang Wang, Dandan Zang, Yucheng Wang
      Pages 183-194
    9. Franziska Glass, Mizuki Takenaka
      Pages 195-205
    10. You Wang, David S. Letham, Peter C. L. John, Ren Zhang
      Pages 225-234
  4. Experimental Approaches to Study Protein-Protein and Protein-DNA Interactions in Plants and Human Cells

    1. Front Matter
      Pages 243-243
    2. Shino Goto-Yamada, Kazumi Hikino, Mikio Nishimura, Tsuyoshi Nakagawa, Shoji Mano
      Pages 245-258
    3. Delphine Masschaele, Sarah Gerlo, Irma Lemmens, Sam Lievens, Jan Tavernier
      Pages 269-278
    4. Alfonso Muñoz, M. Mar Castellano
      Pages 279-287
    5. Zhenyuan Tang, Yoshinori Takahashi
      Pages 289-296
    6. Marta García-León, Elisa Iniesto, Vicente Rubio
      Pages 297-309
    7. Pilar Lasierra, Salomé Prat
      Pages 311-322
    8. Bjørk Ditlev Larsen, Martin Rønn Madsen, Ronni Nielsen, Susanne Mandrup
      Pages 335-352
  5. Back Matter
    Pages 353-355

About this book


This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions.  The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab. 

Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.


Y2H system Protein-protein interactions Gene expression Yeast biology Protein-DNA interaction Plant and mammalian cells

Editors and affiliations

  • Luis Oñate-Sánchez
    • 1
  1. 1.Centro de Biotecnología y Genómica de PlantasUniversidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPMMadridSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7870-0
  • Online ISBN 978-1-4939-7871-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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