Tissue Proteomics

Methods and Protocols

  • Minnie M. Sarwal
  • Tara K. Sigdel

Part of the Methods in Molecular Biology book series (MIMB, volume 1788)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Achim Treumann, Pawel Palmowski, Wing Chiu Tong, Julie Taggart, Nick Morrice, G. Nicholas Europe-Finner et al.
    Pages 1-9
  3. Nicole Valenzuela, Nwe Nwe Soe, Fang Li, Xiaohai Zhang, Yi-Ping Jin, Elaine F. Reed
    Pages 23-41
  4. Tara K. Sigdel, Carrie D. Nicora, Wei-Jun Qian, Minnie M. Sarwal
    Pages 63-72
  5. François-Ludovic Sauvage, Sébastien Passeron, Pierre Marquet
    Pages 113-121
  6. Yuqian Gao, Hui Wang, Carrie D. Nicora, Tujin Shi, Richard D. Smith, Tara K. Sigdel et al.
    Pages 145-156
  7. Kelly J. Shields, Changgong Wu
    Pages 243-250
  8. Yi Chen, David Britton, Elizabeth R. Wood, Stephen Brantley, Michelle Fournier, Marek Wloch et al.
    Pages 251-268
  9. Paul D. Piehowski, Rui Zhao, Ronald J. Moore, Geremy Clair, Charles Ansong
    Pages 269-277
  10. Yvonne Pasing, Armin Schniers, Terkel Hansen
    Pages 289-296
  11. Rémi Longuespée, Deborah Alberts, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, Edwin De Pauw
    Pages 297-312
  12. Back Matter
    Pages 313-320

About this book


This book presents a compilation of methods that detail improved protein and peptide sample preparation and identification. Chapters guide readers through methods for depletion of myofibril-associated proteins, peptide sample preparation in urinary proteomics, purification of targeted proteins from native tissues, fractionation strategies for protein analysis, and GeLC-MS as a sample preparation method for sample preparation for proteomics using minimal amount of tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Tissue Proteomics: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.


myofibril-associated proteins urinary proteomics mass limited tissue samples double isotopologue peptide protein quantification GeLC-MS

Editors and affiliations

  • Minnie M. Sarwal
    • 1
  • Tara K. Sigdel
    • 2
  1. 1.Professor, Department of Surgery Medicine and Pediatrics Director, Kidney-Pancreas Transplant ProgramUniversity of California San FranciscoSan FranciscoUSA
  2. 2.Asst. Professor, Department of SurgeryUniversity of California San FranciscoSan FranciscoUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7852-6
  • Online ISBN 978-1-4939-7854-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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