Methods and Protocols

  • Germain Rousselet

Part of the Methods in Molecular Biology book series (MIMB, volume 1784)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Aoife Kelly, Aleksander M. Grabiec, Mark A. Travis
    Pages 1-11
  3. Chandrayana Mukherjee, Christine Hale, Subhankar Mukhopadhyay
    Pages 13-28 Open Access
  4. Anaïs Assouvie, Lisa P. Daley-Bauer, Germain Rousselet
    Pages 29-33
  5. Dominic De Nardo, Dhan V. Kalvakolanu, Eicke Latz
    Pages 35-49
  6. Calum C. Bain, Stephen J. Jenkins
    Pages 51-67
  7. Kathleen Grabert, Barry W. McColl
    Pages 77-86 Open Access
  8. R. Ian Cumming, Yen-Rei A. Yu
    Pages 99-109
  9. Elizabeth Dalby
    Pages 111-117
  10. Xuan Huang, Yong Li, Mingui Fu, Hong-Bo Xin
    Pages 119-126
  11. Linda Roback, Lisa P. Daley-Bauer
    Pages 127-134
  12. Mourad Aribi
    Pages 135-149
  13. Fernando Montaño, Sergio Grinstein, Roni Levin
    Pages 151-163
  14. Anna Mularski, Florence Marie-Anaïs, Julie Mazzolini, Florence Niedergang
    Pages 165-175
  15. Germain Rousselet
    Pages 177-186
  16. Andrea-Anneliese Keller, Marten B. Maeß, Michael Schnoor, Berith Scheiding, Stefan Lorkowski
    Pages 187-195
  17. Lewis Taylor, Carlota Recio, David R. Greaves, Asif J. Iqbal
    Pages 197-214
  18. Adrian Achuthan, Ming-Chin Lee, Reem Saleh, Andrew J. Fleetwood, John A. Hamilton, Andrew D. Cook
    Pages 215-223
  19. Li Hua, Jiayuan Shi, Leonard D. Shultz, Guangwen Ren
    Pages 243-258
  20. Stéphanie G. Moreno
    Pages 259-262
  21. Jiayuan Shi, Li Hua, Danielle Harmer, Peishan Li, Guangwen Ren
    Pages 263-275
  22. Back Matter
    Pages 277-278

About this book


This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.

The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via


phenotyping chlodronate Chromatin immunoprecipitation inflammasome Influenza

Editors and affiliations

  • Germain Rousselet
    • 1
  1. 1.CEA/DRF/Jacob/iRCM/LRTS, INSERM U967Fontenay aux RosesFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7836-6
  • Online ISBN 978-1-4939-7837-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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