Adipose-Derived Stem Cells

Methods and Protocols

  • Bruce A. Bunnell
  • Jeffrey M. Gimble

Part of the Methods in Molecular Biology book series (MIMB, volume 1773)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Joel A. Aronowitz, Ryan A. Lockhart, Cloe S. Hakakian
    Pages 11-19
  3. Christina L. Rettinger, Kai P. Leung, Rodney K. Chan
    Pages 21-30
  4. Anne-Laure Hafner, Tala Mohsen-Kanson, Christian Dani
    Pages 31-39
  5. Anoosha Forghani, Ram Devireddy
    Pages 41-51
  6. Pascal Morissette Martin, Arthi Shridhar, Claire Yu, Cody Brown, Lauren E. Flynn
    Pages 53-71
  7. Pinar Yilgor Huri, Justin Morrissette-McAlmon, Warren L. Grayson
    Pages 123-135
  8. Gail Kilroy, Marilyn Dietrich, Xiying Wu, Jeffrey M. Gimble, Z. Elizabeth Floyd
    Pages 137-146
  9. Hubert Tseng, Alexes C. Daquinag, Glauco R. Souza, Mikhail G. Kolonin
    Pages 147-154
  10. Jie Li, J. Lowry Curley, Z. Elizabeth Floyd, Xiying Wu, Yuan Di C. Halvorsen, Jeffrey M. Gimble
    Pages 155-165
  11. Francesco Egro, Kacey G. Marra
    Pages 203-213
  12. Satoshi Tajima, Morikuni Tobita, Hiroshi Mizuno
    Pages 261-272
  13. Hui Yu, Margo Emont, Heejin Jun, Jun Wu
    Pages 273-282
  14. Back Matter
    Pages 283-284

About this book


This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application.


regenerative medicine stroma vascular fraction heterogeneous mesenchymal population of cells hematopoietic stem endothelial cells erythrocytes fibroblasts lymphocytes monocyte/macrophages pericytes

Editors and affiliations

  • Bruce A. Bunnell
    • 1
  • Jeffrey M. Gimble
    • 1
    • 2
  1. 1.Center for Stem Cell Research and Regenerative MedicineTulane University School of MedicineNew OrleansUSA
  2. 2.LaCell LLCNew OrleansUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7797-0
  • Online ISBN 978-1-4939-7799-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals