Synthetic Biology

Methods and Protocols

  • Jeffrey Carl Braman

Part of the Methods in Molecular Biology book series (MIMB, volume 1772)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Gene Circuits and Biochemical Pathways

    1. Front Matter
      Pages 1-1
    2. Alberto Sánchez-Pascuala, Pablo I. Nikel, Víctor de Lorenzo
      Pages 3-24
    3. Daniel A. Charlebois, Junchen Diao, Dmitry Nevozhay, Gábor Balázsi
      Pages 25-43
    4. Sofie Lodens, Marilyn De Graeve, Sophie L. K. W. Roelants, Sofie L. De Maeseneire, Wim Soetaert
      Pages 95-123
    5. Takuya Matsumoto, Tsutomu Tanaka, Akihiko Kondo
      Pages 125-136
  3. Genome Editing and Modification

    1. Front Matter
      Pages 137-137
    2. Carsten P. Carstens, Katherine A. Felts, Sarah E. Johns
      Pages 139-150
    3. Jongoh Shin, Namil Lee, Suhyung Cho, Byung-Kwan Cho
      Pages 151-169
    4. Zhen Kang, Wenwen Ding, Peng Jin, Guocheng Du, Jian Chen
      Pages 205-212
    5. Carsten Pohl, László Mózsik, Arnold J. M. Driessen, Roel A. L. Bovenberg, Yvonne I. Nygård
      Pages 213-232
    6. Michael Gapinske, Nathan Tague, Jackson Winter, Gregory H. Underhill, Pablo Perez-Pinera
      Pages 233-248
    7. Keith C. Heyde, Warren C. Ruder
      Pages 249-265
    8. Kaiwen Ivy Liu, Muhammad Nadzim Bin Ramli, Norfala-Aliah Binte Sutrisnoh, Meng How Tan
      Pages 267-288
    9. Naoki Wada, Yasuhiro Kazuki, Kanako Kazuki, Toshiaki Inoue, Kiichi Fukui, Mitsuo Oshimura
      Pages 289-296
    10. Zhong-Tian Zhang, Pablo Jiménez-Bonilla, Seung-Oh Seo, Ting Lu, Yong-Su Jin, Hans P. Blaschek et al.
      Pages 297-325
  4. Genome Language and Computing

    1. Front Matter
      Pages 361-361
    2. Angel Goñi-Moreno, Víctor de Lorenzo
      Pages 363-372
    3. Erin H. Wilson, Chris Macklin, Darren Platt
      Pages 373-398
    4. Vittorio Bartoli, Daniel O. R. Dixon, Thomas E. Gorochowski
      Pages 399-409
    5. Daniel Fu, Shalin Shah, Tianqi Song, John Reif
      Pages 411-417
  5. Molecular Assembly

    1. Front Matter
      Pages 419-419
    2. Zhen Kang, Wenwen Ding, Peng Jin, Guocheng Du, Jian Chen
      Pages 421-428
    3. Hung-En Lai, Simon Moore, Karen Polizzi, Paul Freemont
      Pages 429-444
    4. Rebecca Mullinax, Sarah E. Johns, Denise Rhodes, Vivian Zhang, Nancy McKinney, Katherine A. Felts et al.
      Pages 457-468
    5. Ida Lauritsen, Se Hyeuk Kim, Andreas Porse, Morten H. H. Nørholm
      Pages 469-476
    6. Sabine Gogolok, Ute Köber, Steven M. Pollard
      Pages 477-490
  6. Back Matter
    Pages 491-510

About this book


This detailed book explores a few of the plethora of techniques and applications associated with the unique branch of science known as synthetic biology. Chemists, biologists, and engineers engaged in this multidisciplinary field of study will be guided in the creation and regulation of gene circuits, manipulation of biochemical pathways, genome editing and modification, creating genome language and computing, as well as molecular assembly. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, plus troubleshooting tips and explanations of how to avoid pitfalls, all written by experts who have published their work in peer-reviewed journals. 

Authoritative and practical, Synthetic Biology: Methods and Protocols provides key guidance and ideas for conducting your own synthetic biology projects.


Gene circuits Biomanufacturing Biochemical pathways Genome editing Renewable energy production Genome computing Molecular assembly

Editors and affiliations

  • Jeffrey Carl Braman
    • 1
  1. 1.Agilent Technologies, IncLa JollaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7794-9
  • Online ISBN 978-1-4939-7795-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Chemical Manufacturing
Health & Hospitals
Consumer Packaged Goods