Digital PCR

Methods and Protocols

  • George Karlin-Neumann
  • Francisco  Bizouarn

Part of the Methods in Molecular Biology book series (MIMB, volume 1768)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Background Concepts

    1. Front Matter
      Pages 1-1
    2. Leonardo Pinheiro, Kerry R. Emslie
      Pages 11-24
    3. Alexandra S. Whale, Ana Fernandez-Gonzalez, Alice Gutteridge, Alison S. Devonshire
      Pages 45-65
  3. Absolute Quantification

    1. Front Matter
      Pages 67-67
    2. David Dobnik, Bjørn Spilsberg, Alexandra Bogožalec Košir, Dejan Štebih, Dany Morisset, Arne Holst-Jensen et al.
      Pages 69-98
    3. Yiping Cao, Meredith R. Raith, John F. Griffith
      Pages 127-140
  4. Copy Number Variation

    1. Front Matter
      Pages 141-141
    2. Avery Davis Bell, Christina L. Usher, Steven A. McCarroll
      Pages 143-160
    3. Isaac Garcia-Murillas, Nicholas C. Turner
      Pages 161-172
  5. Rare Mutation and Rare Allele Detection

    1. Front Matter
      Pages 191-191
    2. Yanan Kuang, Allison O’Connell, Adrian G. Sacher, Nora Feeney, Ryan S. Alden, Geoffrey R. Oxnard et al.
      Pages 193-207
    3. Arielle J. Medford, Riaz N. Gillani, Ben Ho Park
      Pages 209-227
    4. Yilun Chen, Anthony M. George, Eleonor Olsson, Lao H. Saal
      Pages 303-322
    5. Yuichiro Miyaoka, Steven J. Mayerl, Amanda H. Chan, Bruce R. Conklin
      Pages 349-362
    6. Ming Yu, Tai J. Heinzerling, William M. Grady
      Pages 363-383
  6. Gene Expression and RNA Quantification

    1. Front Matter
      Pages 385-385
    2. Nolan Kamitaki, Christina L. Usher, Steven A. McCarroll
      Pages 401-422
    3. George Karlin-Neumann, Bin Zhang, Claudia Litterst
      Pages 423-444
    4. Manuela Ferracin, Massimo Negrini
      Pages 445-457
  7. Other Uses of Partitioning

    1. Front Matter
      Pages 475-475
    2. John Regan, George Karlin-Neumann
      Pages 489-512
    3. Andrew T. Ludlow, Dawne Shelton, Woodring E. Wright, Jerry W. Shay
      Pages 513-529
  8. Back Matter
    Pages 555-557

About this book


This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences.”


polymerase chain reaction droplet-based digital PCR infectious disease phenotypes somatic mosaicism genome editing cell therapy haplotyping

Editors and affiliations

  • George Karlin-Neumann
    • 1
  • Francisco  Bizouarn
    • 2
  1. 1.Digital Biology CenterBio-Rad LaboratoriesPleasantonUSA
  2. 2.Digital Biology CenterBio-Rad LaboratoriesPleasantonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7776-5
  • Online ISBN 978-1-4939-7778-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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