Epigenome Editing

Methods and Protocols

  • Albert Jeltsch
  • Marianne G. Rots

Part of the Methods in Molecular Biology book series (MIMB, volume 1767)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. General Reviews

    1. Front Matter
      Pages 1-1
    2. Charlene Babra Waryah, Colette Moses, Mahira Arooj, Pilar Blancafort
      Pages 19-63
    3. Sandra Nitsch, Claudio Mussolino
      Pages 89-109
  3. Topical Reviews

    1. Front Matter
      Pages 111-111
    2. Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, Elizabeth A. Heller
      Pages 113-136
    3. Pavel Bashtrykov, Albert Jeltsch
      Pages 137-146
    4. Sabrina Just, Hildegard Büning
      Pages 147-166
    5. Rutger A. F. Gjaltema, Edda G. Schulz
      Pages 167-185
  4. Delivery Protocols

    1. Front Matter
      Pages 187-187
    2. Tafadzwa Mlambo, Marianna Romito, Tatjana I. Cornu, Claudio Mussolino
      Pages 189-203
    3. Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, Elizabeth A. Heller
      Pages 205-214
    4. Benjamin Pyles, Barbara J. Bailus, Henriette O’Geen, David J. Segal
      Pages 227-239
    5. Jessica A. Kretzmann, Cameron W. Evans, Marck Norret, Pilar Blancafort, K. Swaminathan Iyer
      Pages 241-254
  5. Histone Editing Readout Protocols

  6. DNA Methylation Editing Readout Protocols

    1. Front Matter
      Pages 289-289
    2. Dulce B. Vargas-Landin, Jahnvi Pflüger, Ryan Lister
      Pages 291-298
    3. Magali Kernaleguen, Christian Daviaud, Yimin Shen, Eric Bonnet, Victor Renault, Jean-François Deleuze et al.
      Pages 311-349
    4. Elsa Leitão, Jasmin Beygo, Michael Zeschnigk, Ludger Klein-Hitpass, Marcel Bargull, Sven Rahmann et al.
      Pages 351-366
  7. Gene Expression Readout Protocols

    1. Front Matter
      Pages 383-383
    2. William Beckman, Ilona M. Vuist, Hermannus Kempe, Pernette J. Verschure
      Pages 385-393
    3. Désirée Goubert, Mihály Koncz, Antal Kiss, Marianne G. Rots
      Pages 395-415
  8. Special Application Protocols

    1. Front Matter
      Pages 417-417
    2. Tyler S. Klann, Gregory E. Crawford, Timothy E. Reddy, Charles A. Gersbach
      Pages 447-480
  9. Tafadzwa Mlambo, Marianna Romito, Tatjana I. Cornu, Claudio Mussolino
    Pages E1-E1
  10. Back Matter
    Pages 481-482

About this book


This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.


Histone protein modifications CRISPR dCas9 EpiEditors DNA-binding device Epigenome reprogramming

Editors and affiliations

  • Albert Jeltsch
    • 1
  • Marianne G. Rots
    • 2
  1. 1.Department of BiochemistryInstitute of Biochemistry and Technical BiochemistryStuttgartGermany
  2. 2.Department of Pathology and Medical BiologyUniversity of Groningen, University Medical Center GroningenGroningenThe Netherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7773-4
  • Online ISBN 978-1-4939-7774-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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