Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols

  • Yung-I Lee
  • Edward Chee-Tak Yeung

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Orchid Seeds in Plant Propagation and Conservation

    1. Front Matter
      Pages 1-1
    2. Edward C. Yeung, Yuan-Yuan Li, Yung-I Lee
      Pages 3-26
    3. Lawrence W. Zettler, Laura L. Corey
      Pages 27-59
    4. Yuan-Yuan Li, Hui Wang, Shun-Xing Guo
      Pages 61-69
    5. Philip T. Seaton, Silvério T. Hosomi, Ceci C. Custódio, Timothy R. Marks, Nelson B. Machado-Neto, Hugh W. Pritchard
      Pages 71-98
  3. Orchid Micropropagation

    1. Front Matter
      Pages 99-99
    2. Edward C. Yeung, Jungmin Park, Indra S. Harry
      Pages 101-125
    3. So-Young Park, Yoon-Sun Huh, Kee-Yoeup Paek
      Pages 179-193
    4. Hosakatte Niranjana Murthy, Kee-Yoeup Paek, So-Young Park
      Pages 195-208
  4. Special Techniques

    1. Front Matter
      Pages 225-225
    2. Steven D. Johnson, Lawrence D. Harder
      Pages 227-239
    3. Hua-Ting Chuang, Kuang-Liang Huang, Shan-Te Hsu
      Pages 241-256
    4. Yi-Tien Chiu, Chen Chang
      Pages 257-265
    5. Wagner A. Vendrame
      Pages 283-302
    6. Juntaro Kato, Syoichi Ichihashi
      Pages 303-315
    7. Chin-An Chang
      Pages 331-344
  5. Cell and Molecular Biology and Transformation

    1. Front Matter
      Pages 345-345
    2. Chia-Wen Li, Ming-Tsair Chan
      Pages 367-383
    3. Raffaella Balestrini, Valeria Fochi, Alessandro Lopa, Silvia Perotto
      Pages 385-400
  6. Orchid Propagation and Maintenance

    1. Front Matter
      Pages 401-401
    2. Yung-I Lee
      Pages 403-425
    3. Rong-Show Shen, Yu-Hsin Liao, Kuang-Liang Huang
      Pages 427-446
    4. Jiunn-Feng Su, Shu-Pei Chen, Ting-Fang Hsieh
      Pages 447-460
  7. Showcasing Orchids

    1. Front Matter
      Pages 461-461
    2. Patrick I. Siu
      Pages 477-495
    3. Yuan-Yuan Li, Colin Chan, Corrine Stahl, Edward C. Yeung
      Pages 497-520
  8. Back Matter
    Pages 521-524

About this book


The orchid family is one of the largest families of flowering plants known for their beauty and economic importance.  This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing.
This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation.  The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one’s enjoyment.


Orchid seed biology conservation orchid micropropagation orchid transformation greenhouse techniques

Editors and affiliations

  • Yung-I Lee
    • 1
  • Edward Chee-Tak Yeung
    • 2
  1. 1.Biology DepartmentNational Museum of Natural ScienceTaichungChina
  2. 2.Department of Biological SciencesUniversity of CalgaryCalgaryCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7770-3
  • Online ISBN 978-1-4939-7771-0
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • Buy this book on publisher's site