CpG Islands

Methods and Protocols

  • Tanya Vavouri
  • Miguel A. Peinado

Part of the Methods in Molecular Biology book series (MIMB, volume 1766)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sequence and Other Properties of CpG Islands

    1. Front Matter
      Pages 1-1
    2. Francisco Antequera, Adrian Bird
      Pages 3-13
    3. Hannah K. Long, Nathan R. Rose, Neil P. Blackledge, Robert J. Klose
      Pages 15-29
    4. Cristina Gómez-Martín, Ricardo Lebrón, José L. Oliver, Michael Hackenberg
      Pages 31-47
    5. Humberto J. Ferreira, Manel Esteller
      Pages 49-80
  3. Methods for the Measurement of DNA Methylation at CpG Islands

    1. Front Matter
      Pages 81-81
    2. Ana Monteagudo-Sánchez, Intza Garin, Guiomar Perez de Nanclares, David Monk
      Pages 109-121
    3. Izaskun Mallona, Alberto Sierco, Miguel A. Peinado
      Pages 123-135
    4. Sergio Alonso, Koichi Suzuki, Fumiichiro Yamamoto, Manuel Perucho
      Pages 137-156
    5. Massimiliano Manzo, Christina Ambrosi, Tuncay Baubec
      Pages 157-174
    6. Yulia Medvedeva, Alexander Shershebnev
      Pages 175-194
  4. Chromatin and Related Genomics Methods Used for Understanding the Functional Role of CpG Islands and Other Regulatory Regions

    1. Front Matter
      Pages 195-195
    2. Helena Raurell-Vila, Mireia Ramos-Rodríguez, Lorenzo Pasquali
      Pages 197-208
    3. Masami Ando-Kuri, I. Sarahi M. Rivera, M. Jordan Rowley, Victor G. Corces
      Pages 239-256
    4. Sagar, Josip Stefan Herman, John Andrew Pospisilik, Dominic Grün
      Pages 257-283
    5. Eleni Gelali, Joaquin Custodio, Gabriele Girelli, Erik Wernersson, Nicola Crosetto, Magda Bienko
      Pages 303-333
  5. Eleni Gelali, Joaquin Custodio, Gabriele Girelli, Erik Wernersson, Nicola Crosetto, Magda Bienko
    Pages E1-E1
  6. Back Matter
    Pages 335-340

About this book


This detailed volume examines bioinformatic and molecular biological methods useful to identify and to explore the functions of CpG islands, key navigation points to understand gene regulation in fundamental processes such as development and cell differentiation as well as in diseases like cancer. Beginning with a historical perspective and important properties of CpG islands, the book continues with sections on computational and wet lab methods related to the study of DNA methylation, and in-depth protocols for the analysis of CpG island functional features including epigenetic profiling and chromatin interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, CpG Islands: Methods and Protocols aims to provide readers with the information and methodologies necessary to continue to decipher how a genome’s structure and organization contribute to regulate biological processes.


CpG dinucleotides Gene regulation Bioinformatics Computational methods DNA methylation Epigenetics Chromatin interactions

Editors and affiliations

  • Tanya Vavouri
    • 1
  • Miguel A. Peinado
    • 2
  1. 1.Josep Carreras Leukaemia, Research InstituteBadalonaSpain
  2. 2.IGTP, IMPPCBadalonaSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7767-3
  • Online ISBN 978-1-4939-7768-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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