Intravital Imaging of Dynamic Bone and Immune Systems

Methods and Protocols

  • Masaru Ishii

Part of the Methods in Molecular Biology book series (MIMB, volume 1763)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Junichi Kikuta, Masaru Ishii
    Pages 1-9
  3. Yookyung Jung, Joel A. Spencer, Anthony P. Raphael, Juwell W. Wu, Clemens Alt, Judith R. Runnels et al.
    Pages 11-22
  4. Asuka Sakata, Satoshi Nishimura
    Pages 23-28
  5. Akira Takeda, Masayuki Miyasaka, Eiji Umemoto
    Pages 43-52
  6. Clément Ghigo, Rebecca Gentek, Marc Bajénoff
    Pages 53-61
  7. Stefano Sammicheli, Mirela Kuka, Matteo Iannacone
    Pages 63-74
  8. Jackson LiangYao Li, Chi Ching Goh, Lai Guan Ng
    Pages 87-107
  9. Takashi Hato, Seth Winfree, Pierre C. Dagher
    Pages 129-136
  10. Sayaka Matsumoto, Junichi Kikuta, Masaru Ishii
    Pages 137-143
  11. Ryohei Matsuura, Shigeru Miyagawa, Junichi Kikuta, Masaru Ishii, Yoshiki Sawa
    Pages 145-151
  12. Wataru Ikeda, Ken Sasai, Tsuyoshi Akagi
    Pages 153-163
  13. Back Matter
    Pages 175-175

About this book


This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own. 


Living cells Cellular dynamic movement Osteoclasts Bone cells Multi-photon excitation fluorescent microscopy

Editors and affiliations

  • Masaru Ishii
    • 1
  1. 1.Department of Immunology and Cell BiologyGraduate School of Medicine, Osaka UniversityOsakaJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7761-1
  • Online ISBN 978-1-4939-7762-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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