Protein Complex Assembly

Methods and Protocols

  • Joseph A. Marsh

Part of the Methods in Molecular Biology book series (MIMB, volume 1764)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Experimental Methods

    1. Front Matter
      Pages 1-1
    2. Brian L. Gilmore, A. Cameron Varano, William Dearnaley, Yanping Liang, Bridget C. Marcinkowski, Madeline J. Dukes et al.
      Pages 45-58
    3. Sandip Kaledhonkar, Ziao Fu, Howard White, Joachim Frank
      Pages 59-71
    4. Jose Luis Ortega-Roldan, Martin Blackledge, Malene Ringkjøbing Jensen
      Pages 73-85
    5. Emeline Barbet-Massin, Eli van der Sluis, Joanna Musial, Roland Beckmann, Bernd Reif
      Pages 87-100
    6. Angelica V. Medina-Cucurella, Timothy A. Whitehead
      Pages 101-121
    7. Johanna C. Scheinost, Thomas G. Gligoris
      Pages 123-132
    8. Elisabetta Boeri Erba, Luca Signor, Mizar F. Oliva, Fabienne Hans, Carlo Petosa
      Pages 133-151
    9. Brent A. Kochert, Roxana E. Iacob, Thomas E. Wales, Alexandros Makriyannis, John R. Engen
      Pages 153-171
    10. Philipp Hoess, Markus Mund, Manuel Reitberger, Jonas Ries
      Pages 237-251
    11. Fabian Hertel, Gary C. H. Mo, Peter Dedecker, Jin Zhang
      Pages 267-277
    12. Anthony M. Pedley, Stephen J. Benkovic
      Pages 279-289
    13. Areli Luna-Rico, Jenny-Lee Thomassin, Olivera Francetic
      Pages 291-305
    14. Paushali Chaudhury, Patrick Tripp, Sonja-Verena Albers
      Pages 307-314
    15. Sylvia Eiler, Nicolas Levy, Benoit Maillot, Julien Batisse, Karine Pradeau Aubreton, Oyindamola Oladosu et al.
      Pages 315-328
    16. Florian Weissmann, Jan-Michael Peters
      Pages 329-343
  3. Computational Methods

    1. Front Matter
      Pages 345-345
    2. Jonathan N. Wells, L. Therese Bergendahl, Joseph A. Marsh
      Pages 347-356
    3. Birgit H. M. Meldal, Sandra Orchard
      Pages 377-390
    4. Florian Goebels, Lucas Hu, Gary Bader, Andrew Emili
      Pages 391-399
    5. Iain H. Moal, Raphael A. G. Chaleil, Paul A. Bates
      Pages 413-428
    6. Aravindan Arun Nadaradjane, Raphael Guerois, Jessica Andreani
      Pages 429-447
    7. Dina Schneidman-Duhovny, Michal Hammel
      Pages 449-473
    8. Loïc Salmon, Logan S. Ahlstrom, James C. A. Bardwell, Scott Horowitz
      Pages 491-504
  4. Elisabetta Boeri Erba, Luca Signor, Mizar F. Oliva, Fabienne Hans, Carlo Petosa
    Pages E1-E1
  5. Back Matter
    Pages 505-506

About this book


This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.


stoichiometry Cryoelectron microscopy mass spectrometry co-translational assembly adhesion

Editors and affiliations

  • Joseph A. Marsh
    • 1
  1. 1.MRC Human Genetics Unit, Institute of Genetics & Molecular MedicineUniversity of EdinburghEdinburghUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7758-1
  • Online ISBN 978-1-4939-7759-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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