Nitric Oxide

Methods and Protocols

  • Alexander Mengel
  • Christian Lindermayr

Part of the Methods in Molecular Biology book series (MIMB, volume 1747)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. NO Treatment and Detection

    1. Front Matter
      Pages 1-1
    2. Emrah Eroglu, Helmut Bischof, Suphachai Charoensin, Markus Waldeck-Weiermaier, Wolfgang F. Graier, Roland Malli
      Pages 23-34
    3. John M. Goodwin, Carol A. Chrestensen, Ellen W. Moomaw
      Pages 35-47
    4. Gracia Montilla-Bascón, Julien Mandon, Frans J. M. Harren, Luis A. J. Mur, Simona M. Cristescu, Elena Prats
      Pages 49-57
    5. Paula Mariela González, Susana Puntarulo
      Pages 59-69
  3. NO Modifications

    1. Front Matter
      Pages 71-71
    2. Alicia Izquierdo-Álvarez, Daniel Tello, J. Daniel Cabrera-García, Antonio Martínez-Ruiz
      Pages 73-87
    3. Heaseung Sophia Chung, Christopher I. Murray, Jennifer E. Van Eyk
      Pages 89-101
    4. Lili Zhang, Pengpeng Shang, Changbao Chen, Jie Zhou, Shuhua Zhu
      Pages 103-111
    5. Sébastien Aimé, Siham Hichami, David Wendehenne, Olivier Lamotte
      Pages 131-141
    6. Damian Guerra, Ian Truebridge, Stephen J. Eyles, Patrick Treffon, Elizabeth Vierling
      Pages 143-160
    7. Mounira Chaki, Beatriz Sánchez-Calvo, Alfonso Carreras, Raquel Valderrama, Juan C. Begara-Morales, Francisco J. Corpas et al.
      Pages 161-169
    8. Adil Hussain, Byung-Wook Yun, Gary J. Loake
      Pages 223-230
    9. Capilla Mata-Pérez, María N. Padilla, Beatriz Sánchez-Calvo, Juan C. Begara-Morales, Raquel Valderrama, Francisco J. Corpas et al.
      Pages 231-239
    10. Rosario Carmona, M. Claros, Juan de Alché
      Pages 241-250
  4. NO Detoxification

    1. Front Matter
      Pages 251-251
    2. Changgong Wu, Tong Liu, Yan Wang, Lin Yan, Chuanlong Cui, Annie Beuve et al.
      Pages 253-266
    3. Tereza Tichá, Lenka Luhová, Marek Petřivalský
      Pages 267-280
    4. Sophie Kneeshaw, Steven H. Spoel
      Pages 281-297
  5. Back Matter
    Pages 299-302

About this book


This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part one looks at NO treatments using gaseous nitric oxide and detection using a NO-sensitive electrode, electron spin resonance, and fluorescence-based NO-sensor proteins. Part two talks about various techniques used to detect and identify NO-dependent modifications such as biotin-switch assay and quantification of s-nitrosated proteins. Part three focuses on the study of s-nitrosothiol homeostasis and denitrosation activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, Nitric Oxide: Methods and Protocols is a valuable resource for anyone interested in learning more about this evolving field.


nitrate reductase xanthine oxidase polyamine oxidase electron spin resonance mass spectrometry

Editors and affiliations

  • Alexander Mengel
    • 1
  • Christian Lindermayr
    • 2
  1. 1.Helmholtz Zentrum München–German Research GmbHCenter for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1Munich-NeuherbergGermany
  2. 2.Helmholtz Zentrum München–German Research GmbHCenter for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1Munich-NeuherbergGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7694-2
  • Online ISBN 978-1-4939-7695-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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