Viral Metagenomics

Methods and Protocols

  • Vitantonio Pantaleo
  • Michela Chiumenti

Part of the Methods in Molecular Biology book series (MIMB, volume 1746)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Annalisa Giampetruzzi, Michela Chiumenti, Angelantonio Minafra, Pasquale Saldarelli
    Pages 27-36
  3. Armelle Marais, Chantal Faure, Bernard Bergey, Thierry Candresse
    Pages 45-53
  4. Marion Cornelissen, Astrid Gall, Antoinette van der Kuyl, Chris Wymant, François Blanquart, Christophe Fraser et al.
    Pages 55-61
  5. Ion Gutiérrez-Aguirre, Denis Kutnjak, Nejc Rački, Matevž Rupar, Maja Ravnikar
    Pages 63-75
  6. Sarah François, Denis Filloux, Emmanuel Fernandez, Mylène Ogliastro, Philippe Roumagnac
    Pages 77-95
  7. Luca Nerva, Giovanna C. Varese, Massimo Turina
    Pages 97-114
  8. Nikoletta Czotter, János Molnár, Réka Pesti, Emese Demián, Dániel Baráth, Tünde Varga et al.
    Pages 115-130
  9. Kris De Jonghe, Annelies Haegeman, Yoika Foucart, Martine Maes
    Pages 131-149
  10. Arnfinn Lodden Økland, Are Nylund, Ali May, Adalberto Costessi, Walter Pirovano
    Pages 151-159
  11. Hanako Shimura, Chikara Masuta, Yasunori Koda
    Pages 161-172
  12. Bachir Balech, Alfonso Monaco, Michele Perniola, Monica Santamaria, Giacinto Donvito, Saverio Vicario et al.
    Pages 173-180
  13. Aysan Ghasemzadeh, Marta Małgorzata ter Haar, Masoud Shams-bakhsh, Walter Pirovano, Vitantonio Pantaleo
    Pages 187-195
  14. Finny S. Varghese, Ronald P. van Rij
    Pages 197-213
  15. Aysan Ghasemzadeh, Marta Małgorzata ter Haar, Masoud Shams-bakhsh, Walter Pirovano, Vitantonio Pantaleo
    Pages E1-E1
  16. Back Matter
    Pages 215-217

About this book


This volume explores the use of viral metagenomics to diagnose known viruses for plant and food production, human and animal health, and identifying viral vectors like insects. The chapters in this book cover topics, such as sRNAs isolation from tissues of grapevines and woody plants, high-resolution screening of arthropod and plant viral communities, identifying new pathogens in fish, detecting viruses in mycorrhizal fungi and their orchid host, and insect virus discovery through metagenomic and cell culture-based approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Viral Metagenomics: Methods and Protocols is a valuable resource for researchers and specialists who are interested in learning more about this evolving field.


deep sequencing Next Generation Sequencing cDNA library preparation bioinformatics siRNAs

Editors and affiliations

  • Vitantonio Pantaleo
    • 1
  • Michela Chiumenti
    • 2
  1. 1.Institute Sustainable Plant Protection of the CNRResearch Unit of BariItaly
  2. 2.Institute Sustainable Plant Protection of the CNRResearch Unit of BariItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7682-9
  • Online ISBN 978-1-4939-7683-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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