Metabolic Profiling

Methods and Protocols

  • Georgios A. Theodoridis
  • Helen G. Gika
  • Ian D. Wilson

Part of the Methods in Molecular Biology book series (MIMB, volume 1738)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Fundamentals

    1. Front Matter
      Pages 1-1
    2. Helen G. Gika, Georgios A. Theodoridis, Ian D. Wilson
      Pages 3-13
    3. Olga Begou, Helen G. Gika, Georgios A. Theodoridis, Ian D. Wilson
      Pages 15-26
    4. Samantha Riccadonna, Pietro Franceschi
      Pages 27-39
  3. Methods

    1. Front Matter
      Pages 63-63
    2. Christina Virgiliou, Helen G. Gika, Georgios A. Theodoridis
      Pages 65-81
    3. Elizabeth J. Want
      Pages 99-116
    4. Matthaios-Emmanouil P. Papadimitropoulos, Catherine G. Vasilopoulou, Christoniki Maga-Nteve, Maria I. Klapa
      Pages 133-147
    5. Olga Deda, Helen G. Gika, Georgios A. Theodoridis
      Pages 149-157
    6. Petr Hušek, Zdeněk Švagera, Dagmar Hanzlíková, Iva Karlínová, Lucie Řimnáčová, Helena Zahradníčková et al.
      Pages 159-181
  4. Plant/Food Applications

    1. Front Matter
      Pages 193-193
    2. Evangelia Ralli, Maria Amargianitaki, Efi Manolopoulou, Maria Misiak, Georgios Markakis, Sofia Tachtalidou et al.
      Pages 203-211
    3. Panagiotis Arapitsas, Fulvio Mattivi
      Pages 225-235
  5. Life Science Applications

    1. Front Matter
      Pages 237-237
    2. Panagiotis A. Vorkas, M. R. Abellona U, Jia V. Li
      Pages 239-260
    3. Federico Ponzetto, Julien Boccard, Raul Nicoli, Tiia Kuuranne, Martial Saugy, Serge Rudaz
      Pages 261-278
    4. Aikaterina Siopi, Vassilis Mougios
      Pages 279-287
  6. Back Matter
    Pages 289-291

About this book


This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.


Metabonomics Metabolomics Food and natural products Biomarker discovery Analytical modes

Editors and affiliations

  • Georgios A. Theodoridis
    • 1
  • Helen G. Gika
    • 2
  • Ian D. Wilson
    • 3
  1. 1.Department of ChemistryAristotle University of ThessalonikiThessalonikiGreece
  2. 2.School of MedicineAristotle University of ThessalonikiThessalonikiGreece
  3. 3.Department of Surgery and CancerImperial College LondonLondonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7642-3
  • Online ISBN 978-1-4939-7643-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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