MicroRNA Protocols

  • Shao-Yao Ying

Part of the Methods in Molecular Biology book series (MIMB, volume 1733)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Shao-Yao Ying, Donald C. Chang, Shi-Lung Lin
    Pages 1-25
  3. Mainak Bose, Suvendra N. Bhattacharyya
    Pages 27-39
  4. Juliano Da Silveira, Gabriella M. Andrade, Felipe Perecin, Flávio Vieira Meireles, Quinton A. Winger, Gerrit J. Bouma
    Pages 53-63
  5. Jason Ioannidis, Judith Risse, F. Xavier Donadeu
    Pages 65-77
  6. Max Hübner, Pedro A. F. Galante, Simone Kreth, Ludwig Christian Hinske
    Pages 87-92
  7. Shi-Lung Lin, Shao-Yao Ying
    Pages 107-126
  8. Shi-Lung Lin, Donald C. Chang, Shao-Yao Ying
    Pages 173-180
  9. Andres Stucky, Xuelian Chen, Jiang F. Zhong
    Pages 215-223
  10. Alyne Simões, Zujian Chen, Yan Zhao, Lin Chen, Virgilia Macias, Luisa A. DiPietro et al.
    Pages 225-237
  11. Shi-Lung Lin, Shin-Ju E. Chang, Shao-Yao Ying
    Pages 239-254
  12. Back Matter
    Pages 317-319

About this book


This volume focuses on the analysis of miRNA and its many components such as targets and expression profiling, regulation of gene expression, miRNA detection in biofluids, and its application in cancer and wound healing. The chapters in this book explore topics that’s cover biogenesis, isolation and profiling of miRNA, exosomal miRNAs, and the study of the functional significance of miRNAs. The chapters also cover screening miRNAs in mR-302/367 induced iPSCs, exosomal miRNAs from iPSC-derived cardiomyocytes proliferation, and the recently discovered sugar-like RNA glycylglycerins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, MicroRNA Protocols, Third Edition is a valuable resource that provides researchers with a comprehensive study of miRNAs and its numerous components and uses.


Non-coding RNAs miRNA High-Throughput Expression Cell Death Drosophila cell culture stem cells gene silencing

Editors and affiliations

  • Shao-Yao Ying
    • 1
  1. 1.Department of Integrative Anatomical SciencesUSC, Keck School of MedicineLos AngelesUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7600-3
  • Online ISBN 978-1-4939-7601-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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