Methods and Protocols

  • Dietbert Neumann
  • Benoit Viollet

Part of the Methods in Molecular Biology book series (MIMB, volume 1732)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Christopher G. Langendorf, Jonathan S. Oakhill, Bruce E. Kemp
    Pages 15-27
  3. Ravi G. Kurumbail, Graham M. West, Venkatasubramanian Dharmarajan, Kris A. Borzilleri, Jane M. Withka, Jessica Ward et al.
    Pages 29-55
  4. Fiona A. Fyffe, Simon A. Hawley, Alexander Gray, D. Grahame Hardie
    Pages 69-86
  5. Brendan Gongol, Traci Marin, David A. Johnson, John Y.-J. Shyy
    Pages 99-109
  6. Arpit Jain, Valentin Roustan, Wolfram Weckwerth, Ingo Ebersberger
    Pages 111-142
  7. Pascual Sanz, Rosa Viana, Maria Adelaida Garcia-Gimeno
    Pages 143-157
  8. Jonathan S. Oakhill, John W. Scott, Toby A. Dite
    Pages 159-169
  9. Adrien Grenier, Pierre Sujobert, Séverine Olivier, Hélène Guermouche, Johanna Mondésir, Olivier Kosmider et al.
    Pages 171-194
  10. Biplab Dasgupta, William Seibel
    Pages 195-202
  11. Simon A. Hawley, Fiona A. Fyffe, Fiona M. Russell, Graeme J. Gowans, D. Grahame Hardie
    Pages 239-253
  12. Takafumi Miyamoto, Elmer Rho, Allen Kim, Takanari Inoue
    Pages 255-272
  13. Claudia Marinangeli, Jérome Kluza, Philippe Marchetti, Luc Buée, Valérie Vingtdeux
    Pages 289-305
  14. Sarah J. Mancini, Ian P. Salt
    Pages 307-319
  15. Florence Mailleux, Christophe Beauloye, Jean-Luc Balligand, Sandrine Horman, Luc Bertrand
    Pages 321-342
  16. Joost J. F. P. Luiken, Dietbert Neumann, Jan F. C. Glatz, Will A. Coumans, Dipanjan Chanda, Miranda Nabben
    Pages 343-361
  17. Sarah Krieg, Bernhard Lüscher, Jörg Vervoorts, Marc Dohmen
    Pages 373-391
  18. Chen-Song Zhang, Mengqi Li, Yue Zong, Sheng-Cai Lin
    Pages 393-411
  19. Aida Martinez-Sanchez, Marie-Sophie Nguyen-Tu, Isabelle Leclerc, Guy A. Rutter
    Pages 413-431
  20. Patricia Seoane-Collazo, Miguel López
    Pages 433-448
  21. Renee Rao, Kazuhiro Omi, Roshan Rajani, Hui Li, Nuria M. Pastor-Soler
    Pages 449-464
  22. Sébastien Denanglaire, Tiphène Pirnay, Oberdan Leo, Fabienne Andris
    Pages 465-476
  23. Nicholas D. LeBlond, Morgan D. Fullerton
    Pages 477-493
  24. Swenja Kröller-Schön, Andreas Daiber, Eberhard Schulz
    Pages 495-506
  25. Katrin Spengler, Silke Große, Nderim Kryeziu, Anne Knierim, Regine Heller
    Pages 519-537
  26. Marine Theret, Linda Gsaier, Sabrina Ben Larbi, Michèle Weiss-Gayet, Rémi Mounier
    Pages 539-549
  27. Diana Moreira, Jérôme Estaquier, Anabela Cordeiro-da-Silva, Ricardo Silvestre
    Pages 551-563
  28. Arash Yavari, Dhruv Sarma, Eduardo B. Sternick
    Pages 581-619
  29. Back Matter
    Pages 621-626

About this book


This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.


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Editors and affiliations

  • Dietbert Neumann
    • 1
  • Benoit Viollet
    • 2
  1. 1.Department of Pathology CARIM School for Cardiovascular DiseasesMaastricht UniversityMaastrichtThe Netherlands
  2. 2.Department EMD, Inserm, U1016, Institut Cochin, CNRS, UMR8104Université Paris DescartesParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7597-6
  • Online ISBN 978-1-4939-7598-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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