Clinical Metabolomics

Methods and Protocols

  • Martin Giera

Part of the Methods in Molecular Biology book series (MIMB, volume 1730)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Clinical Metabolomics and Lipidomics

    1. Front Matter
      Pages 1-1
    2. Julijana Ivanisevic, Aurelien Thomas
      Pages 3-28
    3. Bart Everts
      Pages 29-42
  3. LC-MS-Based Metabolomics

    1. Front Matter
      Pages 43-43
    2. Romanas Chaleckis, Shama Naz, Isabel Meister, Craig E. Wheelock
      Pages 45-58
    3. Jesmond Dalli, Romain A. Colas, Mary E. Walker, Charles N. Serhan
      Pages 59-72
    4. Miroslav Lísa, Michal Holčapek
      Pages 73-82
    5. Lennart J. van Winden, Olaf van Tellingen, Huub H. van Rossum
      Pages 93-102
    6. Sabrina Krautbauer, Gerhard Liebisch
      Pages 103-110
    7. Madlen Reinicke, Susen Becker, Uta Ceglarek
      Pages 111-121
    8. Amy A. Rand, Patrick O. Helmer, Bora Inceoglu, Bruce D. Hammock, Christophe Morisseau
      Pages 123-133
    9. Bo Burla, Sneha Muralidharan, Markus R. Wenk, Federico Torta
      Pages 135-162
    10. Lars F. Eggers, Dominik Schwudke
      Pages 163-174
  4. GC-MS-Based Metabolomics

    1. Front Matter
      Pages 237-237
    2. Carole Migné, Stéphanie Durand, Estelle Pujos-Guillot
      Pages 239-246
    3. Lisa R. Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, Martin Giera
      Pages 247-256
    4. Lisa R. Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, Martin Giera
      Pages 257-265
    5. Fabien Riols, Justine Bertrand-Michel
      Pages 267-275
  5. CE-MS-Based Metabolomics

    1. Front Matter
      Pages 293-293
    2. Karina Trevisan Rodrigues, Marina Franco Maggi Tavares, Ann Van Schepdael
      Pages 305-313
  6. NMR-Based Metabolomics

    1. Front Matter
      Pages 315-315
    2. Hye Kyong Kim, Sarantos Kostidis, Young Hae Choi
      Pages 317-328
  7. MALDI-Based Techniques and Mass Spectrometry Imaging of Clinical Samples

    1. Front Matter
      Pages 343-343
    2. Benjamin Balluff, Liam A. McDonnell
      Pages 345-357
  8. Study Design, Data Analysis, and Bioinformatics

    1. Front Matter
      Pages 359-359
    2. Rianne Haumann, Hein W. Verspaget
      Pages 361-370
  9. Back Matter
    Pages 385-389

About this book


This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. The methodologies explored here form the core of several very promising initiatives evolving around personalized health care and precision medicine, which can be seen as complimentary to the field of clinical chemistry and aid the aforementioned field with novel disease markers and diagnostic patterns. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Clinical Metabolomics: Methods and Protocols aims to serve as the basis for successful communication between scientists from several fields, including chemists, biologist, bioinformaticians, and clinicians, ultimately leading to effective study design and completion.


Liquid chromatography–mass spectrometry Clinical chemistry Personalized health care Precision medicine Disease markers Gas chromatography–mass spectrometry

Editors and affiliations

  • Martin Giera
    • 1
  1. 1.Center for Proteomics and MetabolomicsLeiden University Medical CenterLeidenThe Netherlands

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7591-4
  • Online ISBN 978-1-4939-7592-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Chemical Manufacturing
Health & Hospitals
Consumer Packaged Goods