The Surfaceome

Methods and Protocols

  • Kenneth R. Boheler
  • Rebekah L. Gundry

Part of the Methods in Molecular Biology book series (MIMB, volume 1722)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Discovery-Based Approaches to Surfaceome Content

    1. Front Matter
      Pages 1-1
    2. Yulanda M. Williamson, Jennifer Whitmon, Rolieria West-Deadwyler, Hercules Moura, Adrian R. Woolfitt, Jon Rees et al.
      Pages 3-20
    3. Ankit Sinha, Simona Principe, Javier Alfaro, Alex Ignatchenko, Vladimir Ignatchenko, Thomas Kislinger
      Pages 91-102
  3. Targeted Approaches for Surfaceome Content

  4. Cell-Based Functional Analyses Related to Surfaceome Content

    1. Front Matter
      Pages 165-165
    2. Gordon F. Tomaselli, Federica Farinelli
      Pages 167-184
    3. Vladimir Khristov, Qin Wan, Ruchi Sharma, Mostafa Lotfi, Arvydas Maminishkis, Kapil Bharti
      Pages 223-247
    4. Teresa Massam-Wu, Stuart A. Cain, Cay M. Kielty
      Pages 249-260
    5. Prasenjit Bose, Chen Yu Huang, Jeroen Eyckmans, Christopher S. Chen, Daniel H. Reich
      Pages 303-328
  5. Computational Approaches in Surfaceome Studies

  6. Back Matter
    Pages 345-347

About this book


This volume provides readers with the latest techniques and tools to assess modifications and functions of the surfaceome. The chapters in this book are divided into 4 sections: discovery-based approaches to surfaceome content; targeted approaches for surfaceome content; cell-based function analyses related to surfaceome content; and computational approaches in surfaceome studies. Section 1 focuses on discovery-based approaches for cataloging surfaceome content that analyses the surfaceome of bacteria, avian embryos, and mammalian systems. Section 2 discusses methods that over-express specific targets in Sf9 cells and generate bi-specific antibodies for targeting cancer and somatic cells. Section 3 explores voltage dependent sodium channels and high-content electrophysiological analyses. The final section looks at the new web-based platform known as targets-search. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and thorough, Surfaceome: Methods and Protocols assists in the study of cell surface protein biology and function. It is a valuable resource for all researchers interested in this field.


plasma membrane cell surface proteins antibodies lipids and glycans immunophenotyping markers avian embryos and mammalian systems Cell Surface Capture Technology n-glycoproteins G protein couple receptors CXCR4 fluid transport Targets-search bioinformatics

Editors and affiliations

  • Kenneth R. Boheler
    • 1
  • Rebekah L. Gundry
    • 2
  1. 1.School of Biomedical Sciences, LKS Faculty of MedicineThe University of Hong KongHong Kong, SARChina
  2. 2.Department of BiochemistryMedical College of WisconsinMilwaukeeUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7551-8
  • Online ISBN 978-1-4939-7553-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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