Peptidomics

Methods and Strategies

  • Michael Schrader
  • Lloyd Fricker

Part of the Methods in Molecular Biology book series (MIMB, volume 1719)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Overview and Basic Techniques

    1. Front Matter
      Pages 1-1
    2. Elva Fridjonsdottir, Anna Nilsson, Henrik Wadensten, Per E. Andrén
      Pages 41-49
    3. Kazuki Sasaki, Takashi Tsuchiya, Tsukasa Osaki
      Pages 51-58
    4. Mikel Azkargorta, Iraide Escobes, Ibon Iloro, Felix Elortza
      Pages 59-70
    5. Bruce R. Southey, Elena V. Romanova, Sandra L. Rodriguez-Zas, Jonathan V. Sweedler
      Pages 71-96
  3. Quantitative Methods in Peptidomics

    1. Front Matter
      Pages 119-119
    2. Kurt Boonen, Wouter De Haes, Joris Van Houtven, Rik Verdonck, Geert Baggerman, Dirk Valkenborg et al.
      Pages 141-159
    3. Sayani Dasgupta, Leandro M. Castro, Alexandre K. Tashima, Lloyd Fricker
      Pages 161-174
    4. Thomas Otto Kunz, Jiangtian Chen, Megha, Christian Wegener
      Pages 175-185
  4. Diverse Applied Protocols and Outlook

    1. Front Matter
      Pages 197-197
    2. Kristien A. Van Camp, Geert Baggerman, Ronny Blust, Steven J. Husson
      Pages 241-246

About this book

Introduction

This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls.

Authoritative and practical, Peptidomics:Methods and Strategies provides useful guidance for studies in the rapidly growing field of peptidomics. 

Keywords

proteomics liquid chromatography mass spectrometry enzymatic carbohydrates

Editors and affiliations

  • Michael Schrader
    • 1
  • Lloyd Fricker
    • 2
  1. 1.Department of Biotechnology and BioinformaticsWeihenstephan-Tr. University of Applied SciencesFreisingGermany
  2. 2.Department of Molecular PharmacologyAlbert Einstein College of MedicineBronxUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-7537-2
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7536-5
  • Online ISBN 978-1-4939-7537-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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