Traumatic and Ischemic Injury

Methods and Protocols

  • Binu Tharakan

Part of the Methods in Molecular Biology book series (MIMB, volume 1717)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Juan C. Mira, Dina C. Nacionales, Tyler J. Loftus, Ricardo Ungaro, Brittany Mathias, Alicia M. Mohr et al.
    Pages 1-15
  3. Michael R. Williamson, Roseleen F. John, Frederick Colbourne
    Pages 17-25
  4. Himakarnika Alluri, Chinchusha Anasooya Shaji, Matthew L. Davis, Binu Tharakan
    Pages 37-52
  5. Travis M. Doggett, Jared J. Tur, Natascha G. Alves, Sarah Y. Yuan, Srinivas M. Tipparaju, Jerome W. Breslin
    Pages 61-81
  6. Damon Klebe, Loretta Iniaghe, Sherrefa Burchell, Cesar Reis, Onat Akyol, Jiping Tang et al.
    Pages 83-91
  7. Katie Wiggins-Dohlvik, Binu Tharakan
    Pages 93-100
  8. Zhaobin Xu, Kevin E. McElhanon, Eric X. Beck, Noah Weisleder
    Pages 145-153
  9. Jason A. Justice, Russell M. Sanchez
    Pages 155-159
  10. Yugeesh R. Lankadeva, Junko Kosaka, Roger G. Evans, Clive N. May
    Pages 207-218
  11. Ellaine Salvador, Malgorzata Burek, Carola Y. Förster
    Pages 219-227
  12. Bobby Darnell Robinson, Chinchusha Anasooya Shaji, Angela Lomas, Binu Tharakan
    Pages 237-242
  13. Back Matter
    Pages 243-245

About this book


This volume focuses on procedures for the development and application of several research animal models and in vitro methods that allow researchers to gain insight into the underlying cellular, biochemical, and physiological mechanisms involved in traumatic and ischemic injury. The chapters in this book discuss topics, such as animal models and techniques commonly used to study traumatic brain injury, hemorrhagic shock, ischemic and hemorrhagic strokes, sepsis, burn injury, hind limb ischemia, myocardial-ischemia-reperfusion injury, intracranial pressure, global hypoxia-induced perinatal seizures models, and in vitro models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Traumatic and Ischemic Injury: Methods and Protocols is a valuable resource for novices with limited experience to help them initiate new research projects, and established researchers to help them identify comparable approaches and strategies to their studies in this field.


animal model brain injury hemorrhagic stroke sepsis models in vitro models microvascular permeability murine model barrier dysfunction

Editors and affiliations

  • Binu Tharakan
    • 1
  1. 1.Department of SurgeryTexas A&M University Health Science Center, College of Medicine, Baylor Scott and White Research InstituteTempleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7524-2
  • Online ISBN 978-1-4939-7526-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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