Innate Immune Activation

Methods and Protocols

  • Dominic De Nardo
  • Christine M De Nardo

Part of the Methods in Molecular Biology book series (MIMB, volume 1714)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Karin Pelka, Dominic De Nardo
    Pages 1-18
  3. Thomas Ulas, Joachim L. Schultze, Marc Beyer
    Pages 19-40
  4. Moritz M. Gaidt, Francesca Rapino, Thomas Graf, Veit Hornung
    Pages 57-66
  5. Orna Ernst, Sharat J. Vayttaden, Iain D. C. Fraser
    Pages 67-78
  6. Yunhao Tan, Jonathan C. Kagan
    Pages 79-95
  7. Yamel Cardona Gloria, Eicke Latz, Dominic De Nardo
    Pages 97-117
  8. Olaf-Oliver Wolz, Manfred Koegl, Alexander N. R. Weber
    Pages 119-130
  9. Florian Hoss, Verena Rolfes, Mariana R. Davanso, Tarcio T. Braga, Bernardo S. Franklin
    Pages 149-165
  10. Julien Moretti, Nicolas Vabret, J. Magarian Blander
    Pages 167-190
  11. Vincent Compan, Pablo Pelegrín
    Pages 191-197
  12. Roland Felix Dreier, José Carlos Santos, Petr Broz
    Pages 199-213
  13. Annika Frauenstein, Felix Meissner
    Pages 215-227
  14. Stephanie A. Conos, Lisa M. Lindqvist, James E. Vince
    Pages 229-236
  15. Tan A. Nguyen, Lachlan Whitehead, Ken C. Pang
    Pages 237-247
  16. Back Matter
    Pages 249-251

About this book


This volume presents standard approaches and the most recent technical advances used to study innate immune activation. Chapters detail the assessment of macrophage activation, measuring innate immune responses to bacterial viability, quantification of secreted proteins, reporter systems, protocols examining specific innate immune activation by TLRs, RLRs, cGAS, and inflammasomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Innate Immune Activation: Methods and Protocols aims to be a useful and informative reference tool for further study into this vital field.


NFB activation macrophage activation CRISPR endosomal TLRs NFB

Editors and affiliations

  • Dominic De Nardo
    • 1
  • Christine M De Nardo
    • 2
  1. 1.Inflammation DivisionThe Walter & Eliza Hall Institute of Medical ResearchParkvilleAustralia
  2. 2.Chemical Biology DivisionThe Walter & Eliza Hall Institute of Medical ResearchParkvilleAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7518-1
  • Online ISBN 978-1-4939-7519-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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