DNA Methylation Protocols

  • Jörg Tost

Part of the Methods in Molecular Biology book series (MIMB, volume 1708)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Introduction

  3. Global DNA Methylation Levels

    1. Front Matter
      Pages 47-47
    2. Agustin F. Fernandez, Luis Valledor, Fernando Vallejo, Maria Jesús Cañal, Mario F. Fraga
      Pages 49-58
    3. Nathalie Beaujean, Juliette Salvaing, Nur Annies Abd Hadi, Sari Pennings
      Pages 59-80
  4. Genome-Wide DNA Methylation Analysis

    1. Front Matter
      Pages 81-81
    2. Christian Daviaud, Victor Renault, Florence Mauger, Jean-François Deleuze, Jörg Tost
      Pages 83-104
    3. Dieter Weichenhan, Qi Wang, Andrew Adey, Stephan Wolf, Jay Shendure, Roland Eils et al.
      Pages 105-122
    4. Karolina A. Aberg, Robin F. Chan, Linying Xie, Andrey A. Shabalin, Edwin J. C. G. van den Oord
      Pages 171-189
    5. John M. Greally
      Pages 191-207
    6. Xiaoyun Xing, Bo Zhang, Daofeng Li, Ting Wang
      Pages 209-246
    7. Jaroslav Jelinek, Justin T. Lee, Matteo Cesaroni, Jozef Madzo, Shoudan Liang, Yue Lu et al.
      Pages 247-265
    8. Fides D. Lay, Theresa K. Kelly, Peter A. Jones
      Pages 267-284
    9. Clare Stirzaker, Jenny Z. Song, Aaron L. Statham, Susan J. Clark
      Pages 285-302
    10. Michael C. Wu, Pei-Fen Kuan
      Pages 303-330
  5. Analysis of Highly Multiplexed Target Regions

    1. Front Matter
      Pages 331-331
    2. H. Kiyomi Komori, Sarah A. LaMere, Traver Hart, Steven R. Head, Ali Torkamani, Daniel R. Salomon
      Pages 333-348
    3. Martyna Adamowicz, Klio Maratou, Timothy J. Aitman
      Pages 349-363
    4. Dinh Diep, Nongluk Plongthongkum, Kun Zhang
      Pages 365-382
    5. Jennifer Wendt, Heidi Rosenbaum, Todd A. Richmond, Jeffrey A. Jeddeloh, Daniel L. Burgess
      Pages 383-405
    6. Gabriel Beikircher, Walter Pulverer, Manuela Hofner, Christa Noehammer, Andreas Weinhaeusel
      Pages 407-424
  6. Locus-Specific DNA Methylation Analysis

    1. Front Matter
      Pages 425-425
    2. Florence Busato, Emelyne Dejeux, Hafida El abdalaoui, Ivo Glynne Gut, Jörg Tost
      Pages 427-445
    3. João Ramalho-Carvalho, Rui Henrique, Carmen Jerónimo
      Pages 447-472
    4. Mihaela Campan, Daniel J. Weisenberger, Binh Trinh, Peter W. Laird
      Pages 497-513
    5. Cathy B. Moelans, Lilit Atanesyan, Suvi P. Savola, Paul J. van Diest
      Pages 537-549
    6. Dianna Hussmann, Lise Lotte Hansen
      Pages 551-571
    7. Susan M. Mitchell, Keith N. Rand, Zheng-Zhou Xu, Thu Ho, Glenn S. Brown, Jason P. Ross et al.
      Pages 587-601
  7. DNA Methylation Analysis of Specific Biological Samples

    1. Front Matter
      Pages 603-603
    2. Maria Jung, Glen Kristiansen, Dimo Dietrich
      Pages 621-641
  8. Hydroxymethylation

    1. Front Matter
      Pages 643-643
    2. Miao Yu, Dali Han, Gary C. Hon, Chuan He
      Pages 645-663
    3. Kristina Kirschner, Felix Krueger, Anthony R. Green, Tamir Chandra
      Pages 665-678
  9. Back Matter
    Pages 697-704

About this book


This third edition volume expands on the previous editions with a comprehensive update on the available technologies required to successfully perform DNA methylation analysis. The different technologies discussed in this book analyze the global DNA methylation contents, comprehensive analyses using various NGS based methods for genome-wide DNA methylation analysis, along with precise quantification of DNA methylation levels on single CpG positions. The chapters in this book are divided into 7 parts: an introduction to the field along with tips on study design and data analysis; global DNA methylation levels; genome-wide DNA methylation analysis; highly multiplexed target regions; locus-specific DNA methylation analysis; DNA methylation analysis of specific biological samples; and hydroxymethylation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reag
ents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, DNA Methylation Protocols, Third Edition is a valuable resource for postdoctoral investigators and research scientists who work with different aspects of genetics, and cellular and molecular biology, as well as clinicians who are involved in diagnostics or treatment of diseases with epigenetic components.


DNA cytosines gene expression chromatin remodeling molecular assays pyrosequencing single CpG positions methylation-specific PCR

Editors and affiliations

  • Jörg Tost
    • 1
  1. 1.Laboratory for Epigenetics and EnvironmentCentre National de Recherche en Génomique Humaine, CEA—Institut de Biologie Francois JacobEvryFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7479-5
  • Online ISBN 978-1-4939-7481-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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