Bacterial Multidrug Exporters

Methods and Protocols

  • Akihito Yamaguchi
  • Kunihiko Nishino

Part of the Methods in Molecular Biology book series (MIMB, volume 1700)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Structural Analysis of Bacterial Multidrug Exporters

    1. Front Matter
      Pages 1-1
    2. Tsukasa Kusakizako, Yoshiki Tanaka, Christopher J. Hipolito, Hiroaki Suga, Osamu Nureki
      Pages 37-57
    3. Dijun Du, Zhao Wang, Wah Chiu, Ben F. Luisi
      Pages 71-81
    4. Frank Jaenecke, Yoshiko Nakada-Nakura, Kumar Nagarathinam, Satoshi Ogasawara, Kehong Liu, Yunhon Hotta et al.
      Pages 97-109
  3. Biochemical and Bioengineering Analysis of Bacterial Multidrug Exporters

  4. Computational Analysis of Bacterial Multidrug Exporters

    1. Front Matter
      Pages 177-177
    2. Pierpaolo Cacciotto, Venkata K. Ramaswamy, Giuliano Malloci, Paolo Ruggerone, Attilio V. Vargiu
      Pages 179-219
    3. Liping Li, Sasha G. Tetu, Ian T. Paulsen, Karl A. Hassan
      Pages 221-235
  5. Biomedical Approach for Bacterial Multidrug Exporters

    1. Front Matter
      Pages 237-237
    2. Jingjing Sun, Ziqing Deng, Danny Ka Chun Fung, Aixin Yan
      Pages 253-268
    3. Mark K. Haynes, Matthew Garcia, Ryan Peters, Anna Waller, Pietro Tedesco, Oleg Ursu et al.
      Pages 293-318
  6. Advanced Technologies Expected for Application to Multidrug Efflux Transport Studies

    1. Front Matter
      Pages 319-319
    2. Rikiya Watanabe, Naoki Soga, Shin-ya Ohdate, Hiroyuki Noji
      Pages 321-330
    3. Ryota Iino, Shouichi Sakakihara, Yoshimi Matsumoto, Kunihiko Nishino
      Pages 331-341
  7. Back Matter
    Pages 353-355

About this book


This detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria. Beginning with protocols for the structural analysis of bacterial multidrug exporters, the book continues with sections on biochemical and bioengineering analysis, computational analysis, biomedical approaches, as well as advanced technologies expected to be applied to multidrug efflux transport studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bacterial Multidrug Exporters: Methods and Protocols serves as an ideal guide to

this fast moving field of study.


Multidrug-resistant pathogens Bacterial tolerance against antibiotics Drug resistance Crystal structures Membrane proteins Multidrug efflux mechanisms

Editors and affiliations

  • Akihito Yamaguchi
    • 1
  • Kunihiko Nishino
    • 2
  1. 1.Institute of Scientific and Industrial ResearchOsaka UniversityIbarakiJapan
  2. 2.Graduate School of Pharmaceutical SciencesOsaka UniversitySuitaJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7452-8
  • Online ISBN 978-1-4939-7454-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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