Methods and Protocols

  • Joyce A. Lloyd

Part of the Methods in Molecular Biology book series (MIMB, volume 1698)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Matthew P. Parker, Kenneth R. Peterson
    Pages 37-65
  3. Skye C. McIver, Kyle J. Hewitt, Xin Gao, Charu Mehta, Jing Zhang, Emery H. Bresnick
    Pages 67-89
  4. Laura F. Bennett, Chang Liao, Robert F. Paulson
    Pages 91-102
  5. Jia Hao Yeo, Matthew P. Cosgriff, Stuart T. Fraser
    Pages 133-152
  6. Theodosia Kalfa, Kathleen E. McGrath
    Pages 175-192
  7. Xiuli An, Lixiang Chen
    Pages 193-203
  8. Alyson S. Smith, Roberta B. Nowak, Velia M. Fowler
    Pages 205-228
  9. Jinfen J. Yik, Merlin Crossley, Kate G. R. Quinlan
    Pages 245-257
  10. Anna Kovilakath, Safa Mohamad, Farrah Hermes, Shou Zhen Wang, Gordon D. Ginder, Joyce A. Lloyd
    Pages 259-274
  11. Back Matter
    Pages 293-298

About this book


This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to analyze circulating red blood cell parameters, as well as progenitor cell status, stage of differentiation, and enucleation. Methods for the study of erythroid cell gene regulation using chromatin immunoprecipitation and chromatin conformation capture are detailed. Furthermore, protocols are provided to examine stress erythropoiesis, erythroblastic islands, and erythroblast structure. The collection also includes reviews on the available mouse models of erythropoiesis, as well as on future considerations for good manufacturing practice in order to translate the manufacture of erythrocytes to the clinic. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Erythropoiesis: Methods and Protocols provides a definitive source for molecular and cellular biology protocols to study erythropoiesis and to move this vital field forward.


Animal models Red blood cells Progenitor cell status Erythroid cells Chromatin immunoprecipitation (ChIP) Erythroblast structure Erythrocytes

Editors and affiliations

  • Joyce A. Lloyd
    • 1
  1. 1.Department of Human & Molecular GeneticsVirginia Commonwealth UniversityRichmondUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media LLC 2018
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-7427-6
  • Online ISBN 978-1-4939-7428-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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